MICAL-L1 is required for cargo protein delivery to the cell surface.

Autor:	Sikora R; Université de Paris, Inserm U1016-CNRS UMR 8104, Institut Cochin, Paris, France., Bun P; Université de Paris, Institute of Psychiatry and Neuroscience of Paris (IPNP), INSERM U1266, Membrane Traffic in Healthy & Diseased Brain, Paris, France.; Université de Paris, Institute of Psychiatry and Neuroscience of Paris (IPNP), INSERM U1266, NeurImag Imaging facility, 75014 Paris, France., Danglot L; Université de Paris, Institute of Psychiatry and Neuroscience of Paris (IPNP), INSERM U1266, Membrane Traffic in Healthy & Diseased Brain, Paris, France.; Université de Paris, Institute of Psychiatry and Neuroscience of Paris (IPNP), INSERM U1266, NeurImag Imaging facility, 75014 Paris, France., Alqabandi M; Laboratoire Physico Chimie Curie, Institut Curie, PSL Research University, CNRS, UMR168, 75005, Paris, France., Bassereau P; Laboratoire Physico Chimie Curie, Institut Curie, PSL Research University, CNRS, UMR168, 75005, Paris, France., Niedergang F; Université de Paris, Inserm U1016-CNRS UMR 8104, Institut Cochin, Paris, France., Galli T; Université de Paris, Institute of Psychiatry and Neuroscience of Paris (IPNP), INSERM U1266, Membrane Traffic in Healthy & Diseased Brain, Paris, France.; GHU PARIS psychiatrie & neurosciences, F-75014 Paris, France., Zahraoui A; Université de Paris, Inserm U1016-CNRS UMR 8104, Institut Cochin, Paris, France.; Université de Paris, Institute of Psychiatry and Neuroscience of Paris (IPNP), INSERM U1266, Membrane Traffic in Healthy & Diseased Brain, Paris, France.
Jazyk:	angličtina
Zdroj:	Biology open [Biol Open] 2021 Jun 15; Vol. 10 (6). Date of Electronic Publication: 2021 Jun 08.
DOI:	10.1242/bio.058008
Abstrakt:	Secreted proteins are transported along intracellular route from the endoplasmic reticulum through the Golgi before reaching the plasma membrane. Small GTPase Rab and their effectors play a key role in membrane trafficking. Using confocal microscopy, we showed that MICAL-L1 was associated with tubulo-vesicular structures and exhibited a significant colocalization with markers of the Golgi apparatus and recycling endosomes. Super resolution STORM microscopy suggested at the molecular level, a very close association of MICAL-L1 and microdomains in the Golgi cisternae. Using a synchronized secretion assay, we report that the shRNA-mediated depletion of MICAL-L1 impaired the delivery of a subset of cargo proteins to the cell surface. The process of membrane tubulation was monitored in vitro, and we observe that recombinant MICAL-L1-RBD domain may contribute to promote PACSINs-mediated membrane tubulation. Interestingly, two hydrophobic residues at the C-terminus of MICAL-L1 appeared to be important for phosphatidic acid binding, and for association with membrane tubules. Our results reveal a new role for MICAL-L1 in cargo delivery to the plasma membrane. Competing Interests: Competing interests The authors declare no competing or financial interests. (© 2021. Published by The Company of Biologists Ltd.)
Databáze:	MEDLINE
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