Relation between micro- and nanostructure features and biological properties of the decellularized rat liver.
| Autor: | Bobrova MM; Laboratory of Bionanotechnologies, Academician V.I. Shumakov National Medical Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation, 123182 Moscow, Russia., Safonova LA; Laboratory of Bionanotechnologies, Academician V.I. Shumakov National Medical Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation, 123182 Moscow, Russia., Efimov AE; Laboratory of Bionanotechnologies, Academician V.I. Shumakov National Medical Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation, 123182 Moscow, Russia.; SNOTRA LLC., 121205 Moscow, Russia., Iljinsky IM; Laboratory of Bionanotechnologies, Academician V.I. Shumakov National Medical Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation, 123182 Moscow, Russia., Agapova OI; Laboratory of Bionanotechnologies, Academician V.I. Shumakov National Medical Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation, 123182 Moscow, Russia., Agapov II; Laboratory of Bionanotechnologies, Academician V.I. Shumakov National Medical Research Center of Transplantology and Artificial Organs, Ministry of Health of the Russian Federation, 123182 Moscow, Russia. |
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| Jazyk: | angličtina |
| Zdroj: | Biomedical materials (Bristol, England) [Biomed Mater] 2021 Jun 09; Vol. 16 (4). Date of Electronic Publication: 2021 Jun 09. |
| DOI: | 10.1088/1748-605X/ac058b |
| Abstrakt: | Organ decellularization is one of the promising technologies of regenerative medicine, which allows obtaining cell-free extracellular matrix (ECM), which provide preservation of the composition, architecture, vascular network and biological activity of the ECM. The method of decellularization opens up wide prospects for its practical application not only in the field of creating full-scale bioengineered structures, but also in the manufacture of vessels, microcarriers, hydrogels, and coatings. The main goal of our work was the investigation of structure and biological properties of lyophilized decellularized Wistar rat liver fragments (LDLFs), as well as we assessed the regenerative potential of the obtained ECM. We obtained decellularized liver of a Wistar rat, the vascular network and the main components of the ECM of tissue were preserved. H&E staining of histological sections confirmed the removal of cells. DNA content of ECM is equal to 0.7% of native tissue DNA content. Utilizing scanning probe nanotomogrphy method, we showed sinuous, rough topography and highly nanoporous structure of ECM, which provide high level of mouse 3T3 fibroblast and Hep-G (© 2021 IOP Publishing Ltd.) |
| Databáze: | MEDLINE |
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