Resolving Human Lactation Heterogeneity Using Single Milk-Derived Cells, a Resource at the Ready.

Autor: Martin Carli JF; Section of Nutrition, Denver Anschutz Medical Campus Department of Pediatrics, University of Colorado, Aurora, CO, 80045, USA. jayne.martincarli@cuanschutz.edu., Trahan GD; Section of Hematology, Oncology, and Bone Marrow Transplant, Denver Anschutz Medical Campus Department of Pediatrics, University of Colorado, Aurora, CO, 80045, USA., Rudolph MC; Department of Physiology, Oklahoma University Health Sciences Center, Oklahoma City, OK, 73104, USA.
Jazyk: angličtina
Zdroj: Journal of mammary gland biology and neoplasia [J Mammary Gland Biol Neoplasia] 2021 Mar; Vol. 26 (1), pp. 3-8. Date of Electronic Publication: 2021 Jun 07.
DOI: 10.1007/s10911-021-09489-0
Abstrakt: Single cell RNA sequencing (scRNAseq) of human milk-derived cells (HMDCs) makes highly detailed analyses of the biology of human lactation possible. We explore this powerful application as an exciting tool to inspect the cellular composition of human milk. We point out some important challenges unique to this approach and highlight the importance of collaborations between biologists and well-trained bioinformaticians to utilize these data to their maximum potential. We extend this focus by discussing the first two such studies that describe HMDCs via scRNAseq and a variety of important questions in the field that warrant attention through further research. The stage is set to apply scRNAseq in human lactation biology, potentially leading to new insights regarding the molecular and cellular diversity of human secretory mammary epithelial cells.
Databáze: MEDLINE
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