Stress modulation as a means to improve yeasts for lignocellulose bioconversion.

Autor: Brandt BA; Department of Microbiology, Stellenbosch University, Stellenbosch, South Africa., Jansen T; Department of Microbiology, Stellenbosch University, Stellenbosch, South Africa., Volschenk H; Department of Microbiology, Stellenbosch University, Stellenbosch, South Africa., Görgens JF; Department of Process Engineering, Stellenbosch University, Stellenbosch, South Africa., Van Zyl WH; Department of Microbiology, Stellenbosch University, Stellenbosch, South Africa., Den Haan R; Department of Biotechnology, University of the Western Cape, Bellville, South Africa. rdenhaan@uwc.ac.za.
Jazyk: angličtina
Zdroj: Applied microbiology and biotechnology [Appl Microbiol Biotechnol] 2021 Jun; Vol. 105 (12), pp. 4899-4918. Date of Electronic Publication: 2021 Jun 07.
DOI: 10.1007/s00253-021-11383-y
Abstrakt: The second-generation (2G) fermentation environment for lignocellulose conversion presents unique challenges to the fermentative organism that do not necessarily exist in other industrial fermentations. While extreme osmotic, heat, and nutrient starvation stresses are observed in sugar- and starch-based fermentation environments, additional pre-treatment-derived inhibitor stress, potentially exacerbated by stresses such as pH and product tolerance, exist in the 2G environment. Furthermore, in a consolidated bioprocessing (CBP) context, the organism is also challenged to secrete enzymes that may themselves lead to unfolded protein response and other stresses. This review will discuss responses of the yeast Saccharomyces cerevisiae to 2G-specific stresses and stress modulation strategies that can be followed to improve yeasts for this application. We also explore published -omics data and discuss relevant rational engineering, reverse engineering, and adaptation strategies, with the view of identifying genes or alleles that will make positive contributions to the overall robustness of 2G industrial strains. KEYPOINTS: • Stress tolerance is a key driver to successful application of yeast strains in biorefineries. • A wealth of data regarding stress responses has been gained through omics studies. • Integration of this knowledge could inform engineering of fit for purpose strains.
Databáze: MEDLINE
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