Up-regulation of KISS1 as a novel target of Let-7i in melanoma serves as a potential suppressor of migration and proliferation in vitro.

Autor: Alkafaji HA; College of medicine, University of Babylon, Babylon, Iraq., Raji A; College of medicine, University of Babylon, Babylon, Iraq., Rahman HS; Department of Physiology, College of Medicine, University of Suleimanyah, Suleimanyah, Iraq., Zekiy AO; Sechenov First Moscow State Medical University, Moscow, Russian Federation., Adili A; Department of Oncology, Tabriz University of Medical Sciences, Tabriz, Iran., Jalili M; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran., Hojjatipour T; Department of Hematology and Blood Transfusion, Students Research Centre, School of Allied Medicine, Tehran University of Medical Sciences, Tehran, Iran., Cid-Arregui A; Targeted Tumor Vaccines Unit, German Cancer Research Center (DKFZ), Heidelberg, Germany., Shomali N; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.; Department of Immunology, Tabriz University of Medical Sciences, Tabriz, Iran., Tarzi S; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran., Tamjidifar R; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran., Heshmati R; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran., Marofi F; Department of Immunology, Tabriz University of Medical Sciences, Tabriz, Iran., Akbari M; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran., Hasanzadeh A; Department of Immunology, Tabriz University of Medical Sciences, Tabriz, Iran., Deljavanghodrati M; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran., Jarahian M; Toxicology and Chemotherapy Unit (G401), German Cancer Research Center, Heidelberg, Germany., Sandoghchian Shotorbani S; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.; Department of Immunology, Tabriz University of Medical Sciences, Tabriz, Iran.
Jazyk: angličtina
Zdroj: Journal of cellular and molecular medicine [J Cell Mol Med] 2021 Jul; Vol. 25 (14), pp. 6864-6873. Date of Electronic Publication: 2021 Jun 06.
DOI: 10.1111/jcmm.16695
Abstrakt: Melanoma is a kind of skin cancer that is begun by the alteration of melanocytes. miRNAs are small non-coding RNA molecules that regulate a variety of biological processes. KISS1, the metastasis-suppressor gene, encodes kisspeptins which inhibits migration and proliferation of cancers. This study was aimed to determine the role of Let-7i and KISS1 in melanoma cell migration and proliferation. At first, the expression of Let-7i and KISS1 was determined in patients with melanoma. In the in vitro part of the study, Let-7i mimics were transfected and the impact of its restoration on target gene expression, proliferation, migration and apoptosis of SK-MEL-3 melanoma cell line was assessed by real-time PCR and Western blotting, MTT assay, wound-healing assay and flow cytometry, respectively. Besides, KISS1 inhibitor siRNA alone and along with Let-7i was transfected to determine their probable correlation. The results revealed that either Let-7i or KISS1 were down-regulated in patients with melanoma. The results obtained from the in vitro part of the study revealed that restoration of Let-7i reduced the expression of metastasis- and proliferation-related target genes. Moreover, it was revealed that up-regulation of Let-7i attenuated migration and proliferation capability of SK-MEL-3 cells. Besides, it was demonstrated that Let-7i restoration induced apoptosis in melanoma cells. More importantly, the KISS1 inhibitor caused a prominent cell migration and proliferation, attenuated by Let-7i re-expression. To sum up, the present study revealed the impressive role of Let-7i restoration along with its correlation with KISS1 on melanoma carcinogenicity which may be applicable in future in vivo studies.
(© 2021 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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