Biogenic Silver Nanoparticles of Clinacanthus nutans as Antioxidant with Antimicrobial and Cytotoxic Effects.

Autor: Chiu HI; Integrative Medicine Cluster, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Bertam, 13200 Kepala Batas, Penang, Malaysia., Che Mood CNA; Integrative Medicine Cluster, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Bertam, 13200 Kepala Batas, Penang, Malaysia., Mohamad Zain NN; Integrative Medicine Cluster, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Bertam, 13200 Kepala Batas, Penang, Malaysia., Ramachandran MR; Department of Chemistry, Faculty of Science, Universiti Malaya, Kuala Lumpur 50603, Malaysia., Yahaya N; Integrative Medicine Cluster, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Bertam, 13200 Kepala Batas, Penang, Malaysia., Nik Mohamed Kamal NNS; Integrative Medicine Cluster, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Bertam, 13200 Kepala Batas, Penang, Malaysia., Tung WH; School of Pharmacy, University of Nottingham Malaysia, Jalan Broga, Semenyih 43500, Malaysia., Yong YK; Department of Human Anatomy, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia., Lee CK; Bioprocess Technology Division, School of Industrial Technology, Universiti Sains Malaysia, Gelugor, 11800 Penang, Malaysia., Lim V; Integrative Medicine Cluster, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Bertam, 13200 Kepala Batas, Penang, Malaysia.
Jazyk: angličtina
Zdroj: Bioinorganic chemistry and applications [Bioinorg Chem Appl] 2021 May 13; Vol. 2021, pp. 9920890. Date of Electronic Publication: 2021 May 13 (Print Publication: 2021).
DOI: 10.1155/2021/9920890
Abstrakt: Silver nanoparticles (AgNPs) previously synthesised using leaf (AgNP-L) and stem (AgNP-S) extracts of Clinacanthus nutans ( C. nutans ) were tested to evaluate antimicrobial, antioxidant, and cytotoxicity activities. The AgNPs showed good inhibition against bacteria, but not fungi. The inhibition results showed the highest activity against Staphylococcus aureus ( S. aureus ) with 11.35 mm (AgNP-L) and 11.52 mm (AgNP-S), while the lowest inhibition was against Escherichia coli ( E. coli ) with 9.22 mm (AgNP-L) and 9.25 mm (AgNP-S) in the disc diffusion method. The same trend of results was noted in the well diffusion method. The IC 50 of AgNP-L and AgNP-S in 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays was 417.05  μ g/mL and 434.60  μ g/mL, as well as 304.31  μ g/mL and 326.83  μ g/mL, respectively. Ferric reducing power (FRAP) assay showed that AgNP-L [872.389  μ mol/L Fe(II)] and AgNP-S [612.770  μ mol/L Fe(II)] exhibited significantly ( p  < 0.05) greater antioxidant activities than leaf extract (CNL) [152.260  μ mol/L Fe(II)] and stem extract (CNS) [110.445  μ mol/L Fe(II)] of C. nutans . The AgNPs were also proven to possess cytotoxic effects on the breast (MCF-7), cervical (HeLa), and colon (HT-29) cancer cells in a dose-dependent manner. AgNP-S and AgNP-L showed significantly ( p  < 0.05) higher cytotoxicity against MCF-7 (117.43  μ g/mL) and HT-29 (78.47  μ g/mL), respectively. In conclusion, the biosynthesised AgNPs from aqueous extract leaves and stem of C. nutans have demonstrated promising potential towards antioxidant, antimicrobial, and cytotoxicity activities.
Competing Interests: The authors declare that they have no conflicts of interest.
(Copyright © 2021 Hock Ing Chiu et al.)
Databáze: MEDLINE
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