Metagenome Assembled Genome of a Novel Verrucomicrobial Methanotroph From Pantelleria Island.

Autor:	Picone N; Department of Microbiology, Institute for Water and Wetland Research (IWWR), Radboud University, Nijmegen, Netherlands., Blom P; Department of Microbiology, Institute for Water and Wetland Research (IWWR), Radboud University, Nijmegen, Netherlands., Hogendoorn C; Department of Microbiology, Institute for Water and Wetland Research (IWWR), Radboud University, Nijmegen, Netherlands., Frank J; Department of Microbiology, Institute for Water and Wetland Research (IWWR), Radboud University, Nijmegen, Netherlands., van Alen T; Department of Microbiology, Institute for Water and Wetland Research (IWWR), Radboud University, Nijmegen, Netherlands., Pol A; Department of Microbiology, Institute for Water and Wetland Research (IWWR), Radboud University, Nijmegen, Netherlands., Gagliano AL; Istituto Nazionale di Geofisica e Vulcanologia, Sezione di Palermo, Palermo, Italy., Jetten MSM; Department of Microbiology, Institute for Water and Wetland Research (IWWR), Radboud University, Nijmegen, Netherlands., D'Alessandro W; Istituto Nazionale di Geofisica e Vulcanologia, Sezione di Palermo, Palermo, Italy., Quatrini P; Department of Biological, Chemical and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, Palermo, Italy., Op den Camp HJM; Department of Microbiology, Institute for Water and Wetland Research (IWWR), Radboud University, Nijmegen, Netherlands.
Jazyk:	angličtina
Zdroj:	Frontiers in microbiology [Front Microbiol] 2021 May 19; Vol. 12, pp. 666929. Date of Electronic Publication: 2021 May 19 (Print Publication: 2021).
DOI:	10.3389/fmicb.2021.666929
Abstrakt:	Verrucomicrobial methanotrophs are a group of aerobic bacteria isolated from volcanic environments. They are acidophiles, characterized by the presence of a particulate methane monooxygenase (pMMO) and a XoxF-type methanol dehydrogenase (MDH). Metagenomic analysis of DNA extracted from the soil of Favara Grande, a geothermal area on Pantelleria Island, Italy, revealed the presence of two verrucomicrobial Metagenome Assembled Genomes (MAGs). One of these MAGs did not phylogenetically classify within any existing genus. After extensive analysis of the MAG, we propose the name of " Candidatus Methylacidithermus pantelleriae" PQ17 gen. nov. sp. nov. The MAG consisted of 2,466,655 bp, 71 contigs and 3,127 predicted coding sequences. Completeness was found at 98.6% and contamination at 1.3%. Genes encoding the pMMO and XoxF-MDH were identified. Inorganic carbon fixation might use the Calvin-Benson-Bassham cycle since all genes were identified. The serine and ribulose monophosphate pathways were incomplete. The detoxification of formaldehyde could follow the tetrahydrofolate pathway. Furthermore, " Ca. Methylacidithermus pantelleriae" might be capable of nitric oxide reduction but genes for dissimilatory nitrate reduction and nitrogen fixation were not identified. Unlike other verrucomicrobial methanotrophs, genes encoding for enzymes involved in hydrogen oxidation could not be found. In conclusion, the discovery of this new MAG expands the diversity and metabolism of verrucomicrobial methanotrophs. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2021 Picone, Blom, Hogendoorn, Frank, van Alen, Pol, Gagliano, Jetten, D’Alessandro, Quatrini and Op den Camp.)
Databáze:	MEDLINE
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