Multidisciplinary approach in the diagnosis of acute leptospirosis in dogs naturally infected by Leptospira interrogans serogroup Icterohaemorrhagiae: A prospective study.

Autor: Paz LN; Bacterial Disease Laboratory, Postgraduate Program in Animal Science in Tropics - Federal University of Bahia, Adhemar de Barros av, 500, 40170-110, Salvador, BA, Brazil., Dias CS; Bacterial Disease Laboratory, Postgraduate Program in Animal Science in Tropics - Federal University of Bahia, Adhemar de Barros av, 500, 40170-110, Salvador, BA, Brazil., Almeida DS; Bacterial Disease Laboratory, Postgraduate Program in Animal Science in Tropics - Federal University of Bahia, Adhemar de Barros av, 500, 40170-110, Salvador, BA, Brazil., Balassiano IT; Bacterial Zoonoses Laboratory, Leptospirosis National Reference Center/Leptospira collection, WHO/PAHO Collaborating Center for Leptospirosis, Department of Bacteriology, Oswaldo Cruz Institute, FIOCRUZ, Brazil av, 4365, 21045-900, Rio de Janeiro, Brazil., Medeiros MA; Laboratory of Recombinant Technology, Bio-Manguinhos, Brazilian Ministry of Health, FIOCRUZ, Av. Brazil, 4365, 21045-900, Rio de Janeiro, Brazil., Costa F; Collective Health Institute, UFBA, Basílio da Gama st, 40110-000, Canela, Salvador, BA, Brazil; Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Ministério da Saúde, Salvador, Brazil; Institute of Integrative Biology, University of Liverpool, Liverpool, UK; Department of Epidemiology of Microbial Disease, Yale School of Public Health, New Haven, CT, USA., Silva DN; Department of Anatomy, Pathology and Veterinary Clinical Medicine, Federal University of Bahia, Salvador, Bahia, Brazil., Reis JN; Department of Clinical and Toxicological Analysis, School of Pharmacy, Federal University of Bahia / UFBA, Salvador, Bahia, Brazil., Estrela-Lima A; Department of Anatomy, Pathology and Veterinary Clinical Medicine, Federal University of Bahia, Salvador, Bahia, Brazil., Hamond C; Bacterial Disease Laboratory, Postgraduate Program in Animal Science in Tropics - Federal University of Bahia, Adhemar de Barros av, 500, 40170-110, Salvador, BA, Brazil., Pinna MH; Bacterial Disease Laboratory, Postgraduate Program in Animal Science in Tropics - Federal University of Bahia, Adhemar de Barros av, 500, 40170-110, Salvador, BA, Brazil. Electronic address: melissahp@ufba.br.
Jazyk: angličtina
Zdroj: Comparative immunology, microbiology and infectious diseases [Comp Immunol Microbiol Infect Dis] 2021 Aug; Vol. 77, pp. 101664. Date of Electronic Publication: 2021 May 07.
DOI: 10.1016/j.cimid.2021.101664
Abstrakt: Leptospirosis, a zoonotic disease with worldwide distribution, is caused by spirochetes of the genus Leptospira. In dogs, this disease is frequently misdiagnosed. Few studies have attempted to associate the detection of Leptospira spp. infection with clinicopathological and renal histopathological findings using a multidisciplinary approach. The present study isolated and characterized Leptospira spp. obtained from naturally infected dogs and described relevant clinical and histopathological findings. Blood and urine were collected from 57 dogs with clinical symptomatology suggestive of leptospirosis; 38 cases were confirmed by PCR in urine or by culture or microscopic agglutination testing (titers ≥800). A total of 12 strains of pathogenic Leptospira were isolated from the studied dogs (seven in blood, four in urine and one in both blood and urine samples). All isolates were characterized as Leptospira interrogans serogroup Icterohaemorrhagiae. Of the confirmed cases, almost one-third of the animals had been vaccinated. Our analysis of laboratory testing revealed that azotemia and proteinuria were statistically significant predictors of infection. The main histopathological findings seen in kidney tissues were necrosis, degeneration, tubular regeneration, mononuclear inflammatory infiltrate and congestion. A multidisciplinary approach involving clinicopathological and histopathological characterization of renal involvement can aid in the identification of acute leptospirosis infection.
(Copyright © 2021 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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