Peroxisomes in the mouse parotid glands: An in-depth morphological and molecular analysis.

Autor: Watermann C; Institute for Anatomy and Cell Biology II, Medical Cell Biology, Justus Liebig University, Giessen D-35385, Germany; Department of Otorhinolaryngology, Head and Neck Surgery, Justus Liebig University, Giessen D-35392, Germany., Meyer MT; Institute for Anatomy and Cell Biology II, Medical Cell Biology, Justus Liebig University, Giessen D-35385, Germany; Department of Otorhinolaryngology, Head and Neck Surgery, Justus Liebig University, Giessen D-35392, Germany., Valerius KP; Institute for Anatomy and Cell Biology II, Medical Cell Biology, Justus Liebig University, Giessen D-35385, Germany., Kleefeldt F; Institute for Anatomy and Cell Biology, Julius Maximilians University, Würzburg D-97070, Germany., Wagner S; Department of Otorhinolaryngology, Head and Neck Surgery, Justus Liebig University, Giessen D-35392, Germany., Wittekindt C; Department of Otorhinolaryngology, Head and Neck Surgery, Justus Liebig University, Giessen D-35392, Germany., Klussmann JP; Department of Otorhinolaryngology, Head and Neck Surgery, Justus Liebig University, Giessen D-35392, Germany; Department of Otorhinolaryngology, Head and Neck Surgery, Medical Faculty, University of Cologne, Cologne D-50931, Germany., Ergün S; Institute for Anatomy and Cell Biology, Julius Maximilians University, Würzburg D-97070, Germany., Baumgart-Vogt E; Institute for Anatomy and Cell Biology II, Medical Cell Biology, Justus Liebig University, Giessen D-35385, Germany., Karnati S; Institute for Anatomy and Cell Biology II, Medical Cell Biology, Justus Liebig University, Giessen D-35385, Germany; Institute for Anatomy and Cell Biology, Julius Maximilians University, Würzburg D-97070, Germany. Electronic address: Srikanth.karnati@uni-wuerzburg.de.
Jazyk: angličtina
Zdroj: Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft [Ann Anat] 2021 Nov; Vol. 238, pp. 151778. Date of Electronic Publication: 2021 Jun 04.
DOI: 10.1016/j.aanat.2021.151778
Abstrakt: Background: The parotid gland is a major salivary gland that has important roles in the digestive and immune system. Peroxisomes are ubiquitous, single-membrane-bound organelles that are present in all eukaryotic cells. Peroxisomes help mediate lipid and reactive oxygen species metabolism, as well as polyunsaturated fatty acid, cholesterol and plasmalogen synthesis. Much of the knowledge on peroxisomes has derived from metabolic organs, however no detailed knowledge is available on peroxisomes in the parotid glands. We thus aimed to comprehensively delineate the localization and characterization of peroxisomal proteins in the murine parotid gland.
Methods: We characterized peroxisomes in the acinar and striated duct cells of the murine parotid gland by fluorescence and electron microscopy, as well as protein and mRNA expression analyses for important peroxisomal genes and proteins.
Results: We found that peroxisomes are present in all cell types of the mouse parotid gland, however, exhibit notable cell-specific differences in their abundance and enzyme content. We also observed that mouse parotid glands contain high levels of peroxisomal β-oxidation enzymes (including Acox1, Mfp2 and Acaa1), catalase and other peroxisomal anti-oxidative enzymes.
Conclusions: This data suggests that peroxisomes are highly abundant in the murine parotid gland and might help to protect against oxidative stress. This comprehensive description of peroxisomes in the parotid gland lays the groundwork for further research concerning their role in the pathogenesis of parotid gland diseases and tumors.
(Copyright © 2021 Elsevier GmbH. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: