Microfluidic electrical cell lysis for high-throughput and continuous production of cell-free varicella-zoster virus.

Autor: Won EJ; Department of Life Science, Gachon University, Seongnam-si, Gyeonggi-do, 13120, Republic of Korea., Thai DA; Department of BioNano Technology, Gachon University, Seongnam-si, Gyeonggi-do, 13120, Republic of Korea., Duong DD; Department of BioNano Technology, Gachon University, Seongnam-si, Gyeonggi-do, 13120, Republic of Korea., Lee NY; Department of BioNano Technology, Gachon University, Seongnam-si, Gyeonggi-do, 13120, Republic of Korea. Electronic address: nylee@gachon.ac.kr., Song YJ; Department of Life Science, Gachon University, Seongnam-si, Gyeonggi-do, 13120, Republic of Korea. Electronic address: songyj@gachon.ac.kr.
Jazyk: angličtina
Zdroj: Journal of biotechnology [J Biotechnol] 2021 Jul 20; Vol. 335, pp. 19-26. Date of Electronic Publication: 2021 Jun 04.
DOI: 10.1016/j.jbiotec.2021.06.006
Abstrakt: Varicella-zoster virus (VZV), the causative agent of varicella and herpes zoster, is highly cell-associated and spreads via cell-to-cell contact in tissue culture. The lack of cell-free VZV hampers studies on VZV biology as well as antiviral and vaccine development. In the present study, a poly(methylmethacrylate) microfluidic device integrated with arrays of microelectrode was fabricated to continuously electrolyse VZV-infected cells to produce cell-free viruses. By designing multiple constrictions and microelectrode arrays, a high electric field is focused on the constricted region of the microchannel to disrupt large numbers of virus-infected cells with high-throughput on a microfluidic platform. Plaque assay and scanning electron microscopy were conducted to quantify and characterize cell-free VZV produced using the microfluidic continuous-flow electrical cell lysis device. The process of microfluidic electrical cell lysis followed by subsequent filtration and virus concentration process yielded a 1.4-2.1 × 10 4 plaque-forming units (PFUs) per mL of cell-free VZV from 7.0 × 10 6 VZV-infected human foreskin fibroblasts (HFF) cells. The high electric field formed inside a microfluidic channel combined with the continuous-flow of virus-infected cells within the microchannel enabled the rapid and efficient production of high-titer cell-free virus in large quantities with relatively low input of the voltage.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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