| Autor: |
Brunner C; Neuro-Electronics Research Flanders, Leuven, Belgium.; VIB, Leuven, Belgium.; Imec, Leuven, Belgium.; Department of Neurosciences, KU Leuven, Leuven, Belgium., Grillet M; Neuro-Electronics Research Flanders, Leuven, Belgium.; VIB, Leuven, Belgium.; Imec, Leuven, Belgium.; Department of Neurosciences, KU Leuven, Leuven, Belgium., Urban A; Neuro-Electronics Research Flanders, Leuven, Belgium.; VIB, Leuven, Belgium.; Imec, Leuven, Belgium.; Department of Neurosciences, KU Leuven, Leuven, Belgium., Roska B; Institute of Molecular and Clinical Ophthalmology Basel, Basel, Switzerland.; University of Basel, Basel, Switzerland.; NCCR Molecular Systems Engineering, Basel, Switzerland., Montaldo G; Neuro-Electronics Research Flanders, Leuven, Belgium. gabriel.montaldo@nerf.be.; VIB, Leuven, Belgium. gabriel.montaldo@nerf.be.; Imec, Leuven, Belgium. gabriel.montaldo@nerf.be.; Department of Neurosciences, KU Leuven, Leuven, Belgium. gabriel.montaldo@nerf.be., Macé E; Brain-Wide Circuits for Behavior Lab, Max Planck Institute of Neurobiology, Martinsried, Germany. emace@neuro.mpg.de. |
| Abstrakt: |
Most brain functions engage a network of distributed regions. Full investigation of these functions thus requires assessment of whole brains; however, whole-brain functional imaging of behaving animals remains challenging. This protocol describes how to follow brain-wide activity in awake head-fixed mice using functional ultrasound imaging, a method that tracks cerebral blood volume dynamics. We describe how to set up a functional ultrasound imaging system with a provided acquisition software (miniScan), establish a chronic cranial window (timing surgery: ~3-4 h) and image brain-wide activity associated with a stimulus at high resolution (100 × 110 × 300 µm and 10 Hz per brain slice, which takes ~45 min per imaging session). We include codes that enable data to be registered to a reference atlas, production of 3D activity maps, extraction of the activity traces of ~250 brain regions and, finally, combination of data from multiple sessions (timing analysis averages ~2 h). This protocol enables neuroscientists to observe global brain processes in mice. |
Full text from SpringerLink