Budding yeast relies on G 1 cyclin specificity to couple cell cycle progression with morphogenetic development.

Autor: Pirincci Ercan D; Chromosome Segregation Laboratory, The Francis Crick Institute, London, UK., Chrétien F; Chromosome Segregation Laboratory, The Francis Crick Institute, London, UK., Chakravarty P; Bioinformatics and Biostatistics Science Technology Platform, The Francis Crick Institute, London, UK., Flynn HR; Proteomics Science Technology Platform, The Francis Crick Institute, London, UK., Snijders AP; Proteomics Science Technology Platform, The Francis Crick Institute, London, UK., Uhlmann F; Chromosome Segregation Laboratory, The Francis Crick Institute, London, UK. frank.uhlmann@crick.ac.uk.
Jazyk: angličtina
Zdroj: Science advances [Sci Adv] 2021 Jun 04; Vol. 7 (23). Date of Electronic Publication: 2021 Jun 04 (Print Publication: 2021).
DOI: 10.1126/sciadv.abg0007
Abstrakt: Two models have been put forward for cyclin-dependent kinase (Cdk) control of the cell cycle. In the qualitative model, cell cycle events are ordered by distinct substrate specificities of successive cyclin waves. Alternatively, in the quantitative model, the gradual rise of Cdk activity from G 1 phase to mitosis leads to ordered substrate phosphorylation at sequential thresholds. Here, we study the relative contributions of qualitative and quantitative Cdk control in Saccharomyces cerevisiae All S phase and mitotic cyclins can be replaced by a single mitotic cyclin, albeit at the cost of reduced fitness. A single cyclin can also replace all G 1 cyclins to support ordered cell cycle progression, fulfilling key predictions of the quantitative model. However, single-cyclin cells fail to polarize or grow buds and thus cannot survive. Our results suggest that budding yeast has become dependent on G 1 cyclin specificity to couple cell cycle progression to essential morphogenetic events.
(Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY).)
Databáze: MEDLINE
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