The impact of thermal processing on the simulated infant gastrointestinal digestion, bactericidal and anti-inflammatory activity of bovine lactoferrin - An in vitro study.

Autor: Goulding DA; School of Food and Nutritional Sciences, University College Cork, Cork, Ireland., Vidal K; Nestlé Research, Nestlé Institute of Health Sciences, Vers-chez-les-Blanc, CH-1000 Lausanne 26, Switzerland., Bovetto L; Nestlé Research, Nestlé Institute of Material Sciences, Vers-chez-les-Blanc, CH-1000 Lausanne 26, Switzerland., O'Regan J; Nestlé Development Centre Nutrition, Askeaton, Co. Limerick, Ireland., O'Brien NM; School of Food and Nutritional Sciences, University College Cork, Cork, Ireland., O'Mahony JA; School of Food and Nutritional Sciences, University College Cork, Cork, Ireland. Electronic address: sa.omahony@ucc.ie.
Jazyk: angličtina
Zdroj: Food chemistry [Food Chem] 2021 Nov 15; Vol. 362, pp. 130142. Date of Electronic Publication: 2021 May 18.
DOI: 10.1016/j.foodchem.2021.130142
Abstrakt: Lactoferrin (LF) is a multifunctional glycoprotein which, when thermally processed, undergoes significant physicochemical changes. The link between such changes and the bioactivity of LF is not well characterised and requires much research. In this work, bovine LF solutions (1%, w/v, protein, pH 7) were thermally processed using high temperature short time conditions (72, 80, 85 or 95 °C with 15 s holding times). Following this, it was shown that LF and heat induced LF aggregates were largely resistant to simulated infant gastric, but not intestinal, digestion. Also, the efficacy of LF bactericidal activity, and inhibition of lipopolysaccharide-induced NF-κB activation were negatively impacted by thermal processing. This study confirmed that the efficacy of LF bio-functionalities was affected by the extent of heat-induced changes in protein structure whereby processing conditions of least severity (i.e. pasteurisation) had the least impact on bioactivity.
(Copyright © 2021 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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