An ex vivo physiologic and hyperplastic vessel culture model to study intra-arterial stent therapies.

Autor: Wang J; Department of Anesthesiology, School of Medicine, Yale University, New Haven, CT, 06519, USA., Kural MH; Department of Anesthesiology, School of Medicine, Yale University, New Haven, CT, 06519, USA., Wu J; Department of Biomedical Engineering, School of Medicine, Yale University, New Haven, CT, 06519, USA., Leiby KL; Department of Biomedical Engineering, School of Medicine, Yale University, New Haven, CT, 06519, USA., Mishra V; Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT, 06269, USA., Lysyy T; Department of Surgery, School of Medicine, Yale University, New Haven, CT, 06519, USA., Li G; Department of Surgery, School of Medicine, Yale University, New Haven, CT, 06519, USA., Luo J; Yale Cardiovascular Research Center, Department of Internal Medicine, School of Medicine, Yale University, New Haven, CT06519, USA., Greaney A; Department of Biomedical Engineering, School of Medicine, Yale University, New Haven, CT, 06519, USA., Tellides G; Department of Surgery, School of Medicine, Yale University, New Haven, CT, 06519, USA., Qyang Y; Yale Cardiovascular Research Center, Department of Internal Medicine, School of Medicine, Yale University, New Haven, CT06519, USA., Huang N; School of Material Science and Engineering, Southwest Jiaotong University, Chengdu, Sichuan, 610031, China., Niklason LE; Department of Anesthesiology, School of Medicine, Yale University, New Haven, CT, 06519, USA; Department of Biomedical Engineering, School of Medicine, Yale University, New Haven, CT, 06519, USA. Electronic address: laura.niklason@yale.edu.
Jazyk: angličtina
Zdroj: Biomaterials [Biomaterials] 2021 Aug; Vol. 275, pp. 120911. Date of Electronic Publication: 2021 May 29.
DOI: 10.1016/j.biomaterials.2021.120911
Abstrakt: Conventional in vitro methods for biological evaluation of intra-arterial devices such as stents fail to accurately predict cytotoxicity and remodeling events. An ex vivo flow-tunable vascular bioreactor system (VesselBRx), comprising intra- and extra-luminal monitoring capabilities, addresses these limitations. VesselBRx mimics the in vivo physiological, hyperplastic, and cytocompatibility events of absorbable magnesium (Mg)-based stents in ex vivo stent-treated porcine and human coronary arteries, with in-situ and real-time monitoring of local stent degradation effects. Unlike conventional, static cell culture, the VesselBRx perfusion system eliminates unphysiologically high intracellular Mg 2+ concentrations and localized O 2 consumption resulting from stent degradation. Whereas static stented arteries exhibited only 20.1% cell viability and upregulated apoptosis, necrosis, metallic ion, and hypoxia-related gene signatures, stented arteries in VesselBRx showed almost identical cell viability to in vivo rabbit models (~94.0%). Hyperplastic intimal remodeling developed in unstented arteries subjected to low shear stress, but was inhibited by Mg-based stents in VesselBRx, similarly to in vivo. VesselBRx represents a critical advance from the current static culture standard of testing absorbable vascular implants.
(Copyright © 2021 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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