Open sandwich fluorescence-linked immunosorbent assay for detection of soy isoflavone glycosides.

Autor: Sakamoto S; Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan. Electronic address: s.sakamoto@phar.kyushu-u.ac.jp., Uchiyama H; Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan., Yusakul G; School of Pharmacy, Walailak University, Nakhon Si Thammarat 80160, Thailand., Kyokong N; Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok 10330, Thailand., Pongkitwitoon B; Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand., Putalun W; Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002, Thailand., Tanaka H; Faculty of Pharmaceutical Sciences, Sanyo-Onoda City University, 1-1-1 Daigaku-dori, Sanyo-Onoda City, 756-0884 Yamaguchi, Japan., Morimoto S; Graduate School of Pharmaceutical Sciences, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
Jazyk: angličtina
Zdroj: Food chemistry [Food Chem] 2021 Nov 01; Vol. 361, pp. 129829. Date of Electronic Publication: 2021 Apr 20.
DOI: 10.1016/j.foodchem.2021.129829
Abstrakt: To detect major soy isoflavone glycosides, namely daidzin (DZ) and genistin (GEN), novel open sandwich fluorescence-linked immunosorbent assay (os-FLISA) was developed by taking advantage of enhanced interactions between variable regions of heavy (VH) and light chain (VL) domains in the presence of an antigen. The VH and VL genes were expressed in Escherichia coli as a chimera protein with green fluorescence protein (AcGFP1) and maltose-binding protein (MBP), respectively. Comprehensive characterization of os-FLISA displayed nearly the same specificity as parental DZ- and GEN-specific monoclonal antibody, demonstrating the potential of the developed assay for detection of both DZ and GEN. Their detectable range in this system exhibited at 0.1-12.5 μg mL -1 . Subsequent validation analysis revealed that os-FLISA was reliable and accurate system for detection of total soy isoflavone glycosides. Notably, this is the first FLISA based on an open sandwich system, which can be employed for the detection of small molecules.
(Copyright © 2021 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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