Optical monitoring of polymerizations in droplets with high temporal dynamic range.

Autor:	Cavell AC; Department of Chemistry, University of Wisconsin-Madison 1101 University Avenue Madison WI 53706 USA rhg@chem.wisc.edu., Krasecki VK; Department of Chemistry, University of Wisconsin-Madison 1101 University Avenue Madison WI 53706 USA rhg@chem.wisc.edu., Li G; Department of Chemistry, Northwestern University 2145 Sheridan Road Evanston IL 60208 USA., Sharma A; School of Chemistry, University of Glasgow Joseph Black Building, University Avenue Glasgow Scotland G12 8QQ UK., Sun H; Department of Chemistry, Northwestern University 2145 Sheridan Road Evanston IL 60208 USA., Thompson MP; Department of Chemistry, Northwestern University 2145 Sheridan Road Evanston IL 60208 USA., Forman CJ; Department of Chemistry, Northwestern University 2145 Sheridan Road Evanston IL 60208 USA., Guo SY; Department of Chemistry, University of Toronto 80 St. George Street Toronto Ontario M5S 3H6 Canada.; Department of Computer Science, University of Toronto 40 St. George Street Toronto Ontario M5S 2E4 Canada., Hickman RJ; Department of Chemistry, University of Toronto 80 St. George Street Toronto Ontario M5S 3H6 Canada.; Department of Computer Science, University of Toronto 40 St. George Street Toronto Ontario M5S 2E4 Canada., Parrish KA; Department of Chemistry, University of Wisconsin-Madison 1101 University Avenue Madison WI 53706 USA rhg@chem.wisc.edu., Aspuru-Guzik A; Department of Chemistry, University of Toronto 80 St. George Street Toronto Ontario M5S 3H6 Canada.; Department of Computer Science, University of Toronto 40 St. George Street Toronto Ontario M5S 2E4 Canada.; Canadian Institute for Advanced Research (CIFAR) Senior Fellow Toronto Ontario M5S 1M1 Canada.; CIFAR Artificial Intelligence Chair, Vector Institute Toronto Ontario M5S 1M1 Canada., Cronin L; School of Chemistry, University of Glasgow Joseph Black Building, University Avenue Glasgow Scotland G12 8QQ UK., Gianneschi NC; Department of Chemistry, Northwestern University 2145 Sheridan Road Evanston IL 60208 USA., Goldsmith RH; Department of Chemistry, University of Wisconsin-Madison 1101 University Avenue Madison WI 53706 USA rhg@chem.wisc.edu.
Jazyk:	angličtina
Zdroj:	Chemical science [Chem Sci] 2020 Feb 04; Vol. 11 (10), pp. 2647-2656. Date of Electronic Publication: 2020 Feb 04.
DOI:	10.1039/c9sc05559b
Abstrakt:	The ability to optically monitor a chemical reaction and generate an in situ readout is an important enabling technology, with applications ranging from the monitoring of reactions in flow, to the critical assessment step for combinatorial screening, to mechanistic studies on single reactant and catalyst molecules. Ideally, such a method would be applicable to many polymers and not require only a specific monomer for readout. It should also be applicable if the reactions are carried out in microdroplet chemical reactors, which offer a route to massive scalability in combinatorial searches. We describe a convenient optical method for monitoring polymerization reactions, fluorescence polarization anisotropy monitoring, and show that it can be applied in a robotically generated microdroplet. Further, we compare our method to an established optical reaction monitoring scheme, the use of Aggregation-Induced Emission (AIE) dyes, and find the two monitoring schemes offer sensitivity to different temporal regimes of the polymerization, meaning that the combination of the two provides an increased temporal dynamic range. Anisotropy is sensitive at early times, suggesting it will be useful for detecting new polymerization "hits" in searches for new reactivity, while the AIE dye responds at longer times, suggesting it will be useful for detecting reactions capable of reaching higher molecular weights. Competing Interests: There are no conflicts to declare. (This journal is © The Royal Society of Chemistry.)
Databáze:	MEDLINE
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