Gene Expression and Survival of Acute Lymphoblastic Leukemia Cells After Allogeneic Transplant.

Autor:	Schenone D; Division of Pediatric Hematology/Oncology, University of Rochester, Rochester, NY, U.S.A., Andolina JR; Division of Pediatric Hematology/Oncology, University of Rochester, Rochester, NY, U.S.A., Rademacher B; Division of Pediatric Hematology/Oncology, University of Rochester, Rochester, NY, U.S.A., Fountaine TJ; Division of Pediatric Hematology/Oncology, University of Rochester, Rochester, NY, U.S.A., Edwards E; Division of Pediatric Hematology/Oncology, University of Rochester, Rochester, NY, U.S.A., Nunez L; Division of Pediatric Hematology/Oncology, University of Rochester, Rochester, NY, U.S.A., Qiu M; Division of Pediatric Hematology/Oncology, University of Rochester, Rochester, NY, U.S.A., Sharma S; Division of Pediatric Hematology/Oncology, University of Rochester, Rochester, NY, U.S.A., Mullen CA; Division of Pediatric Hematology/Oncology, University of Rochester, Rochester, NY, U.S.A. craig_mullen@urmc.rochester.edu.
Jazyk:	angličtina
Zdroj:	Anticancer research [Anticancer Res] 2021 Jun; Vol. 41 (6), pp. 2781-2793.
DOI:	10.21873/anticanres.15059
Abstrakt:	Background/aim: This study explored the mechanisms of the allogeneic graft versus leukemia effect in acute lymphoblastic leukemia (ALL) cells by examining whether they change gene expression in the post-transplant environment containing cytokines and the immunosuppressant cyclosporine, and if such changes affect ALL cell survival. Materials and Methods: RNASeq was used to assess leukemia global gene expression and flow cytometry to measure ALL survival in the presence of T cells, NK cells, cytokines, and cyclosporine. Results: A total of 4,805 genes were differentially expressed. Gene set enrichment analysis demonstrated up-regulation of biological processes related to cytokine responses, control of viral infection, and regulation of leukocyte function including proliferation. Down-regulated genes were related to mesenchymal tissue morphogenesis. ALL cells exposed to cytokines and cyclosporine retained susceptibility to T and NK cell killing, and also exhibited increased cell death without exposure to killer cells. Conclusion: A significant portion of the graft versus leukemia effect may be mediated by cytokines and cyclosporine. (Copyright © 2021 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)
Databáze:	MEDLINE
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