Comparison between Enzyme Immunoassays Performed on Samples of Dried Blood and Serum for Toxoplasmosis Prenatal Screening: Population-based Study.
| Autor: | Marques BA; Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil., Carellos EVM; Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil., Silva VMN; Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil., Pereira FH; Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil., Guerra MRL; Fundação Ezequiel Dias, Belo Horizonte, MG, Brazil., Iturra JAD; Fundação Ezequiel Dias, Belo Horizonte, MG, Brazil., Januário JN; Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil., Andrade GMQ; Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil. |
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| Jazyk: | angličtina |
| Zdroj: | Revista brasileira de ginecologia e obstetricia : revista da Federacao Brasileira das Sociedades de Ginecologia e Obstetricia [Rev Bras Ginecol Obstet] 2021 May; Vol. 43 (5), pp. 351-356. Date of Electronic Publication: 2021 Jun 02. |
| DOI: | 10.1055/s-0041-1730285 |
| Abstrakt: | Objective: Most prenatal screening programs for toxoplasmosis use immunoassays in serum samples of pregnant women. Few studies assess the accuracy of screening tests in dried blood spots, which are of easy collection, storage, and transportation. The goals of the present study are to determine the performance and evaluate the agreement between an immunoassay of dried blood spots and a reference test in the serum of pregnant women from a population-based prenatal screening program for toxoplasmosis in Brazil. Methods: A cross-sectional study was performed to compare the immunoassays Imunoscreen Toxoplasmose IgM and Imunoscreen Toxoplasmose IgG (Mbiolog Diagnósticos, Ltda., Contagem, Minas Gerais, Brazil)in dried blood spots with the enzyme-linked fluorescent assay (ELFA, BioMérieux S.A., Lyon, France) reference standard in the serum of pregnant women from Minas Gerais Congenital Toxoplasmosis Control Program. Results: The dried blood spot test was able to discriminate positive and negative results of pregnant women when compared with the reference test, with an accuracy of 98.2% for immunoglobulin G (IgG), and of 95.8% for immunoglobulin M (IgM). Conclusion: Dried blood samples are easy to collect, store, and transport, and they have a good performance, making this a promising method for prenatal toxoplasmosis screening programs in countries with continental dimensions, limited resources, and a high prevalence of toxoplasmosis, as is the case of Brazil. Competing Interests: The authors have no conflict of interests to declare. (Federação Brasileira de Ginecologia e Obstetrícia. This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://creativecommons.org/licenses/by/4.0/).) |
| Databáze: | MEDLINE |
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