Scale-up of an intensified bioprocess for the expansion of bovine adipose-derived stem cells (bASCs) in stirred tank bioreactors.

Autor: Hanga MP; School of Biosciences, College of Life and Health Sciences, Aston University, Aston Triangle, Birmingham, UK., de la Raga FA; School of Biosciences, College of Life and Health Sciences, Aston University, Aston Triangle, Birmingham, UK., Moutsatsou P; School of Biosciences, College of Life and Health Sciences, Aston University, Aston Triangle, Birmingham, UK., Hewitt CJ; School of Biosciences, College of Life and Health Sciences, Aston University, Aston Triangle, Birmingham, UK., Nienow AW; School of Biosciences, College of Life and Health Sciences, Aston University, Aston Triangle, Birmingham, UK.; Department of Chemical Engineering, University of Birmingham, Edgbaston, Birmingham, UK., Wall I; School of Biosciences, College of Life and Health Sciences, Aston University, Aston Triangle, Birmingham, UK.; Department of Nanobiomedical Science, Institute of Tissue Regeneration Engineering, Dankook University, Cheonan, South Korea.
Jazyk: angličtina
Zdroj: Biotechnology and bioengineering [Biotechnol Bioeng] 2021 Aug; Vol. 118 (8), pp. 3175-3186. Date of Electronic Publication: 2021 Jun 02.
DOI: 10.1002/bit.27842
Abstrakt: Cultivated meat is an emerging field, aiming to establish the production of animal tissue for human consumption in an in vitro environment, eliminating the need to raise and slaughter animals for their meat. To realise this, the expansion of primary cells in a bioreactor is needed to achieve the high cell numbers required. The aim of this study was to develop a scalable, microcarrier based, intensified bioprocess for the expansion of bovine adipose-derived stem cells as precursors of fat and muscle tissue. The intensified bioprocess development was carried out initially in spinner flasks of different sizes and then translated to fully controlled litre scale benchtop bioreactors. Bioprocess intensification was achieved by utilising the previously demonstrated bead-to-bead transfer phenomenon and through the combined addition of microcarrier and medium to double the existing surface area and working volume in the bioreactor. Choosing the optimal time point for the additions was critical in enhancing the cell expansion. A significant fold increase of 114.19 ± 1.07 was obtained at the litre scale in the intensified bioprocess compared to the baseline (**p < .005). The quality of the cells was evaluated pre- and post-expansion and the cells were found to maintain their phenotype and differentiation capacity.
(© 2021 Biotechnology and Bioengineering published by Wiley Periodicals LLC.)
Databáze: MEDLINE
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