| Autor: |
Bukanova J; Functional Synaptology Laboratory, Brain Research Department, Research Center of Neurology, Volokolamskoe Shosse 80, 125367 Moscow, Russia., Solntseva E; Functional Synaptology Laboratory, Brain Research Department, Research Center of Neurology, Volokolamskoe Shosse 80, 125367 Moscow, Russia., Kondratenko R; Functional Synaptology Laboratory, Brain Research Department, Research Center of Neurology, Volokolamskoe Shosse 80, 125367 Moscow, Russia., Kudova E; Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Flemingovo Namesti 2, 160 00 Prague, Czech Republic. |
| Abstrakt: |
Epipregnanolone (3β-hydroxy-5β-pregnan-20-one, Epi) is an endogenous steroid with important physiological effects and high affinity for GABA A receptors. The effect of Epi on GABA-induced chloride current ( I GABA ) in native neurons has hardly been studied. In this work, we studied the influence of Epi on the I GABA in the Purkinje cells of rat cerebellum and pyramidal neurons of rat hippocampus with the patch clamp technique. We showed that Epi is a positive modulator of the I GABA with EC 50 of 5.7 µM in Purkinje cells and 9.3 µM in hippocampal neurons. Epi-induced potentiation of the I GABA was more potent at low vs. high GABA concentrations. Isopregnanolone (3β-hydroxy-5α-pregnan-20-one, Iso) counteracted Epi, reducing its potentiating effect by 2-2.3 times. Flumazenil, a nonsteroidal GABA A receptor antagonist, does not affect the Epi-induced potentiation. Comparison of the potentiating effects of Epi and allopregnanolone (3α-hydroxy-5α-pregnan-20-one, ALLO) showed that ALLO is, at least, a four times more potent positive modulator than Epi. The combined application of ALLO and Epi showed that the effects of these two steroids are not additive. We conclude that Epi has a dual effect on the I GABA increasing the current in the control solution and decreasing the stimulatory effect of ALLO. |
