| Autor: |
Paun IA; Center for Advanced Laser Technologies (CETAL), National Institute for Laser, Plasma and Radiation Physics, RO-077125 Magurele-Ilfov, Romania.; Faculty of Applied Sciences, University Politehnica of Bucharest, RO-060042 Bucharest, Romania., Calin BS; Center for Advanced Laser Technologies (CETAL), National Institute for Laser, Plasma and Radiation Physics, RO-077125 Magurele-Ilfov, Romania.; Faculty of Applied Sciences, University Politehnica of Bucharest, RO-060042 Bucharest, Romania., Mustaciosu CC; Horia Hulubei National Institute for Physics and Nuclear Engineering IFIN-HH, RO-077125 Magurele-Ilfov, Romania.; Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, RO-060042 Bucharest, Romania., Tanasa E; Faculty of Applied Sciences, University Politehnica of Bucharest, RO-060042 Bucharest, Romania.; National Institute for Laser, Plasma and Radiation Physics, RO-077125 Magurele-Ilfov, Romania., Moldovan A; National Institute for Laser, Plasma and Radiation Physics, RO-077125 Magurele-Ilfov, Romania., Niemczyk A; Department of Materials Technology, Faculty of Mechanical Engineering and Mechatronics, West Pomeranian University of Technology in Szczecin, 19 Piastow Ave, 70-310 Szczecin, Poland., Dinescu M; National Institute for Laser, Plasma and Radiation Physics, RO-077125 Magurele-Ilfov, Romania. |
| Abstrakt: |
We report the design and fabrication by laser direct writing via two photons polymerization of innovative hierarchical structures with cell-repellency capability. The structures were designed in the shape of "mushrooms", consisting of an underside (mushroom's leg) acting as a support structure and a top side (mushroom's hat) decorated with micro- and nanostructures. A ripple-like pattern was created on top of the mushrooms, over length scales ranging from several µm (microstructured mushroom-like pillars, MMP) to tens of nm (nanostructured mushroom-like pillars, NMP). The MMP and NMP structures were hydrophobic, with contact angles of (127 ± 2)° and (128 ± 4)°, respectively, whereas flat polymer surfaces were hydrophilic, with a contact angle of (43 ± 1)°. The cell attachment on NMP structures was reduced by 55% as compared to the controls, whereas for the MMP, a reduction of only 21% was observed. Moreover, the MMP structures preserved the native spindle-like with phyllopodia cellular shape, whereas the cells from NMP structures showed a round shape and absence of phyllopodia. Overall, the NMP structures were more effective in impeding the cellular attachment and affected the cell shape to a greater extent than the MMP structures. The influence of the wettability on cell adhesion and shape was less important, the cellular behavior being mainly governed by structures' topography. |
