| Autor: |
Konopleva MV; Federal State Budget Institution 'National Research Centre for Epidemiology and Microbiology Named After Honorary Academician N.F. Gamaleya' of the Ministry of Health of the Russian Federation, 123098 Moscow, Russia., Belenikin MS; Laboratory of Molecular Medical Diagnostics, Moscow Institute of Physics and Technology, State University, 141701 Dolgoprudny, Russia., Shanko AV; Federal State Budget Institution 'National Research Centre for Epidemiology and Microbiology Named After Honorary Academician N.F. Gamaleya' of the Ministry of Health of the Russian Federation, 123098 Moscow, Russia., Bazhenov AI; State Budget Institution 'Research Institute of Emergency Medicine Named After N.V. Sklifosovsky' of the Moscow Department of Healthcare, 129010 Moscow, Russia., Kiryanov SA; Federal State Budget Institution 'National Research Centre for Epidemiology and Microbiology Named After Honorary Academician N.F. Gamaleya' of the Ministry of Health of the Russian Federation, 123098 Moscow, Russia., Tupoleva TA; National Research Center for Hematology, 125167 Moscow, Russia., Sokolova MV; Federal State Budget Institution 'National Research Centre for Epidemiology and Microbiology Named After Honorary Academician N.F. Gamaleya' of the Ministry of Health of the Russian Federation, 123098 Moscow, Russia., Pronin AV; Federal State Budget Institution 'National Research Centre for Epidemiology and Microbiology Named After Honorary Academician N.F. Gamaleya' of the Ministry of Health of the Russian Federation, 123098 Moscow, Russia., Semenenko TA; Federal State Budget Institution 'National Research Centre for Epidemiology and Microbiology Named After Honorary Academician N.F. Gamaleya' of the Ministry of Health of the Russian Federation, 123098 Moscow, Russia., Suslov AP; Federal State Budget Institution 'National Research Centre for Epidemiology and Microbiology Named After Honorary Academician N.F. Gamaleya' of the Ministry of Health of the Russian Federation, 123098 Moscow, Russia. |
| Abstrakt: |
Multiple studies of hepatitis B virus (HBV) genetic variability and its relationship with the disease pathogenesis are currently ongoing, stemming from growing evidence of the clinical significance of HBV mutations. It is becoming increasingly evident that patients with hematologic malignancies may be particularly prone to a higher frequency of such mutations. The present report is the first extensive study of the prevalence of escape mutations in S-HBsAg, performed using isolates from 59 patients from hospital hematology departments with diagnoses of leukemia (n = 32), lymphoma (n = 20), multiple myeloma (n = 3), and non-tumor blood diseases (n = 4). The isolates were serologically examined for the presence of HBV markers and sequenced using either next-generation sequencing (NGS) or Sanger sequencing. Occult hepatitis B was found in 5.1% of cases. Genetic analysis of the region corresponding to S-HBsAg demonstrated an exceptionally high mutation frequency in patients with leukemias (93.4%) and lymphomas (85.0%), along with the prominent mutation heterogeneity. Additionally, more than 15 mutations in one sample were found in patients with leukemias (6.3% of cases) and lymphomas (5.0% of cases). Most of the mutations were clinically significant. The study analyzes the mutation profile of HBV in different oncohematological diseases and the frequency of individual mutations. The data strongly suggest that the NGS method, capable of detecting minor populations of HBV mutations, provides a diagnostic advantage, lays the foundation for the development of screening methods, and allows for the study of the virological and pathogenetic aspects of hepatitis B. |
