| Autor: |
Marro M; ICFO-Institut de Ciencies Fotoniques, The Barcelona Institute of Science and Technology, Castelldefels, 08860 Barcelona, Spain., Rodríguez-Rivero AM; Research and Development B. Braun Surgical, S.A.U. Ctra. de Terrassa, 121, Rubí, 08191 Barcelona, Spain.; Campus de la UAB, Universitat Autònoma de Barcelona, Plaça Cívica, Bellaterra, 08193 Barcelona, Spain., Araujo-Andrade C; ICFO-Institut de Ciencies Fotoniques, The Barcelona Institute of Science and Technology, Castelldefels, 08860 Barcelona, Spain., Fernández-Figueras MT; Departament de Medicina, Facultat de Medicina i Ciències de la Salut, Universitat Internacional de Catalunya, Carrer de la Immaculada, 22, 08017 Barcelona, Spain.; Institut de Recerca Germans Trias i Pujol (IGTP), Camí de les Escoles, s/n, 08916 Badalona, Spain., Pérez-Roca L; Institut de Recerca Germans Trias i Pujol (IGTP), Camí de les Escoles, s/n, 08916 Badalona, Spain., Castellà E; Institut de Recerca Germans Trias i Pujol (IGTP), Camí de les Escoles, s/n, 08916 Badalona, Spain., Navinés J; Institut de Recerca Germans Trias i Pujol (IGTP), Camí de les Escoles, s/n, 08916 Badalona, Spain., Mariscal A; Institut de Recerca Germans Trias i Pujol (IGTP), Camí de les Escoles, s/n, 08916 Badalona, Spain., Julián JF; Campus de la UAB, Universitat Autònoma de Barcelona, Plaça Cívica, Bellaterra, 08193 Barcelona, Spain.; Institut de Recerca Germans Trias i Pujol (IGTP), Camí de les Escoles, s/n, 08916 Badalona, Spain., Turon P; Research and Development B. Braun Surgical, S.A.U. Ctra. de Terrassa, 121, Rubí, 08191 Barcelona, Spain., Loza-Alvarez P; ICFO-Institut de Ciencies Fotoniques, The Barcelona Institute of Science and Technology, Castelldefels, 08860 Barcelona, Spain. |
| Abstrakt: |
Microcalcifications are detected through mammography screening and, depending on their morphology and distribution (BI-RADS classification), they can be considered one of the first indicators of suspicious cancer lesions. However, the formation of hydroxyapatite (HAp) calcifications and their relationship with malignancy remains unknown. In this work, we report the most detailed three-dimensional biochemical analysis of breast cancer microcalcifications to date, combining 3D Raman spectroscopy imaging and advanced multivariate analysis in order to investigate in depth the molecular composition of HAp calcifications found in 26 breast cancer tissue biopsies. We demonstrate that DNA has been naturally adsorbed and encapsulated inside HAp microcalcifications. Furthermore, we also show the encapsulation of other relevant biomolecules in HAp calcifications, such as lipids, proteins, cytochrome C and polysaccharides. The demonstration of natural DNA biomineralization, particularly in the tumor microenvironment, represents an unprecedented advance in the field, as it can pave the way to understanding the role of HAp in malignant tissues. |
