| Autor: |
Raldugina GN; K. A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, 127276 Moscow, Russia., Evsukov SV; K. A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya Street 35, 127276 Moscow, Russia., Bogoutdinova LR; Plant Cell Biology Laboratory, All-Russia Research Institute of Agricultural Biotechnology, Russian Academy of Sciences, Timiryazevskaya Street 42, 127550 Moscow, Russia., Gulevich AA; Plant Cell Engineering Laboratory, All-Russia Research Institute of Agricultural Biotechnology, Timiryazevskaya 42, 127550 Moscow, Russia., Baranova EN; Plant Cell Biology Laboratory, All-Russia Research Institute of Agricultural Biotechnology, Russian Academy of Sciences, Timiryazevskaya Street 42, 127550 Moscow, Russia.; N.V. Tsitsin Main Botanical Garden of Russian Academy of Sciences, 127276 Moscow, Russia. |
| Abstrakt: |
In this study the transgenic lines (TLs) of tobacco ( Nicotiana tabacum L.), which overexpress the heterologous gene encoding the bacterial enzyme choline oxidase were evaluated. The goal of our work is to study the effect of choline oxidase gene expression on the sensitivity of plant tissues to the action of NaCl. The regenerative capacity, rhizogenesis, the amount of photosynthetic pigments and osmotically active compounds (proline and glycine betaine) were assessed by in vitro cell culture methods using biochemical and morphological parameters. Transgenic lines with confirmed expression were characterized by high regeneration capacity from callus in the presence of 200 mmol NaCl, partial retention of viability at 400 mmol NaCl. These data correlated with the implicit response of regenerants and whole plants to the harmful effects of salinity. They turned out to be less sensitive to the presence of 200 mmol NaCl in the cultivation medium, in contrast to the WT plants. |
