Diamond Nanoparticles-Porphyrin mTHPP Conjugate as Photosensitizing Platform: Cytotoxicity and Antibacterial Activity.

Autor: Hurtado CR; Federal Institute of São Paulo (IFSP), São José dos Campos 12223-201, São Paulo, Brazil.; Nanomaterials and Nanotoxicology Laboratory, Institute of Science and Technology, Federal University of São Paulo (UNIFESP), São José dos Campos 12231-280, São Paulo, Brazil.; Peptide Biochemistry Laboratory, Institute of Science and Technology, Federal University of São Paulo (UNIFESP), São José dos Campos 12231-280, São Paulo, Brazil., Hurtado GR; Institute of Science and Technology, São Paulo State University (UNESP), São José dos Campos 12247-004, São Paulo, Brazil.; Institute of Advanced Sea Studies (IEAMAr), São Paulo State University (UNESP), São José dos Campos 12247-004, São Paulo, Brazil., Cena GL; Peptide Biochemistry Laboratory, Institute of Science and Technology, Federal University of São Paulo (UNIFESP), São José dos Campos 12231-280, São Paulo, Brazil., Queiroz RC; Federal Institute of São Paulo (IFSP), São José dos Campos 12223-201, São Paulo, Brazil.; Nanomaterials and Nanotoxicology Laboratory, Institute of Science and Technology, Federal University of São Paulo (UNIFESP), São José dos Campos 12231-280, São Paulo, Brazil., Silva AV; Institute of Medicinal Chemistry, Helmholtz Zentrum München, 30167 Hanover, Germany., Diniz MF; Fundamental Sciences Division, Technological Institute of Aeronautics (ITA), São José dos Campos 12228-900, São Paulo, Brazil., Santos VRD; Bioceramics Laboratory, Institute of Science and Technology, Federal University of São Paulo (UNIFESP), São José dos Campos 12231-280, São Paulo, Brazil., Trava-Airoldi V; Sensors and Materials Associated Laboratory, National Institute for Space Research (INPE), São José dos Campos 12227-010, São Paulo, Brazil., Baptista MDS; Department of Biochemistry, Institute of Chemistry, University of São Paulo (USP), São Paulo 05508-000, São Paulo, Brazil., Tsolekile N; Department of Chemical Sciences, University of Johannesburg, P.O. Box 17011, Doornfontein, Johannesburg 2028, South Africa.; Centre for Nanomaterials Science Research, University of Johannesburg, Johannesburg 2028, South Africa., Oluwafemi OS; Department of Chemical Sciences, University of Johannesburg, P.O. Box 17011, Doornfontein, Johannesburg 2028, South Africa.; Centre for Nanomaterials Science Research, University of Johannesburg, Johannesburg 2028, South Africa., Conceição K; Peptide Biochemistry Laboratory, Institute of Science and Technology, Federal University of São Paulo (UNIFESP), São José dos Campos 12231-280, São Paulo, Brazil., Tada DB; Nanomaterials and Nanotoxicology Laboratory, Institute of Science and Technology, Federal University of São Paulo (UNIFESP), São José dos Campos 12231-280, São Paulo, Brazil.
Jazyk: angličtina
Zdroj: Nanomaterials (Basel, Switzerland) [Nanomaterials (Basel)] 2021 May 25; Vol. 11 (6). Date of Electronic Publication: 2021 May 25.
DOI: 10.3390/nano11061393
Abstrakt: Conjugation of photosensitizers (PS) with nanoparticles has been largely used as a strategy to stabilize PS in the biological medium resulting in photosensitizing nanoparticles of enhanced photoactivity. Herein, (Meso-5, 10, 15, 20-tetrakis (3-hydroxyphenyl) phorphyryn (mTHPP) was conjugated with diamond nanoparticles (ND) by covalent bond. Nanoconjugate ND-mTHPP showed suitable stability in aqueous suspension with 58 nm of hydrodynamic diameter and Zeta potential of -23 mV. The antibacterial activity of ND-mTHPP was evaluated against Escherichia coli for different incubation times (0-24 h). The optimal activity was observed after 2 h of incubation and irradiation (660 nm; 51 J/cm 2 ) performed right after the addition of ND-mTHPP (100 μg/mL) to the bacterial suspension. The inhibitory activity was 56% whereas ampicillin at the same conditions provided only 14% of bacterial growth inhibition. SEM images showed agglomerate of ND-mTHPP adsorbed on the bacterial cell wall, suggesting that the antimicrobial activity of ND-mTHPP was afforded by inducing membrane damage. Cytotoxicity against murine embryonic fibroblast cells (MEF) was also evaluated and ND-mTHPP was shown to be noncytotoxic since viability of cells cultured for 24 h in the presence of the nanoconjugate (100 μg/mL) was 78%. Considering the enhanced antibacterial activity and the absence of cytotoxic effect, it is possible to consider the ND-mTHPP nanoconjugate as promising platform for application in antimicrobial photodynamic therapy (aPDT).
Databáze: MEDLINE