| Autor: |
Luján-Soto E; Departamento de Bioquímica, Facultad de Química, Universidad Nacional Autónoma de Mexico, CdMx 04510, Mexico., Juárez-González VT; Departamento de Bioquímica, Facultad de Química, Universidad Nacional Autónoma de Mexico, CdMx 04510, Mexico., Reyes JL; Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de Mexico, Av. Universidad 2001, Cuernavaca Mor 62210, Mexico., Dinkova TD; Departamento de Bioquímica, Facultad de Química, Universidad Nacional Autónoma de Mexico, CdMx 04510, Mexico. |
| Jazyk: |
angličtina |
| Zdroj: |
International journal of molecular sciences [Int J Mol Sci] 2021 May 18; Vol. 22 (10). Date of Electronic Publication: 2021 May 18. |
| DOI: |
10.3390/ijms22105310 |
| Abstrakt: |
MicroRNAs (miRNAs) are small non-coding RNAs that regulate the accumulation and translation of their target mRNAs through sequence complementarity. miRNAs have emerged as crucial regulators during maize somatic embryogenesis (SE) and plant regeneration. A monocot-specific miRNA, mainly accumulated during maize SE, is zma-miR528. While several targets have been described for this miRNA, the regulation has not been experimentally confirmed for the SE process. Here, we explored the accumulation of zma-miR528 and several predicted targets during embryogenic callus induction, proliferation, and plantlet regeneration using the maize cultivar VS-535. We confirmed the cleavage site for all tested zma-miR528 targets; however, PLC1 showed very low levels of processing. The abundance of zma-miR528 slightly decreased in one month-induced callus compared to the immature embryo (IE) explant tissue. However, it displayed a significant increase in four-month sub-cultured callus, coincident with proliferation establishment. In callus-regenerated plantlets, zma-miR528 greatly decreased to levels below those observed in the initial explant. Three of the target transcripts ( MATE , bHLH , and SOD1a ) showed an inverse correlation with the miRNA abundance in total RNA samples at all stages. Using polysome fractionation, zma-miR528 was detected in the polysome fraction and exhibited an inverse distribution with the PLC1 target, which was not observed at total RNA. Accordingly, we conclude that zma-miR528 regulates multiple target mRNAs during the SE process by promoting their degradation, translation inhibition or both. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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