3D Bioprinting of Model Tissues That Mimic the Tumor Microenvironment.

Autor: Bojin F; Department of Functional Sciences, Victor Babes University of Medicine and Pharmacy Timisoara, 300041 Timisoara, Romania.; OncoGen Institute, 300723 Timisoara, Romania., Robu A; Department of Automation and Applied Informatics, 'Politehnica' University of Timisoara, 300223 Timisoara, Romania., Bejenariu MI; Faculty of Mechanical Engineering, 'Politehnica' University of Timisoara, 300222 Timisoara, Romania., Ordodi V; OncoGen Institute, 300723 Timisoara, Romania., Olteanu E; Department of Functional Sciences, Victor Babes University of Medicine and Pharmacy Timisoara, 300041 Timisoara, Romania.; Department of Microscopic Morphology-Morphopathology, ANAPATMOL Research Center, Victor Babes University of Medicine and Pharmacy Timisoara, 300041 Timisoara, Romania., Cean A; OncoGen Institute, 300723 Timisoara, Romania., Popescu R; Department of Functional Sciences, Victor Babes University of Medicine and Pharmacy Timisoara, 300041 Timisoara, Romania., Neagu M; Department of Functional Sciences, Victor Babes University of Medicine and Pharmacy Timisoara, 300041 Timisoara, Romania.; Center for Modeling Biological Systems and Data Analysis, Victor Babes University of Medicine and Pharmacy Timisoara, 300041 Timisoara, Romania., Gavriliuc O; Department of Functional Sciences, Victor Babes University of Medicine and Pharmacy Timisoara, 300041 Timisoara, Romania.; OncoGen Institute, 300723 Timisoara, Romania., Neagu A; Department of Functional Sciences, Victor Babes University of Medicine and Pharmacy Timisoara, 300041 Timisoara, Romania.; Center for Modeling Biological Systems and Data Analysis, Victor Babes University of Medicine and Pharmacy Timisoara, 300041 Timisoara, Romania.; Department of Physics and Astronomy, University of Missouri, Columbia, MO 65211, USA., Arjoca S; Department of Functional Sciences, Victor Babes University of Medicine and Pharmacy Timisoara, 300041 Timisoara, Romania.; Center for Modeling Biological Systems and Data Analysis, Victor Babes University of Medicine and Pharmacy Timisoara, 300041 Timisoara, Romania., Păunescu V; Department of Functional Sciences, Victor Babes University of Medicine and Pharmacy Timisoara, 300041 Timisoara, Romania.; OncoGen Institute, 300723 Timisoara, Romania.
Jazyk: angličtina
Zdroj: Micromachines [Micromachines (Basel)] 2021 May 09; Vol. 12 (5). Date of Electronic Publication: 2021 May 09.
DOI: 10.3390/mi12050535
Abstrakt: The tumor microenvironment (TME) influences cancer progression. Therefore, engineered TME models are being developed for fundamental research and anti-cancer drug screening. This paper reports the biofabrication of 3D-printed avascular structures that recapitulate several features of the TME. The tumor is represented by a hydrogel droplet uniformly loaded with breast cancer cells (10 6 cells/mL); it is embedded in the same type of hydrogel containing primary cells-tumor-associated fibroblasts isolated from the peritumoral environment and peripheral blood mononuclear cells. Hoechst staining of cryosectioned tissue constructs demonstrated that cells remodeled the hydrogel and remained viable for weeks. Histological sections revealed heterotypic aggregates of malignant and peritumoral cells; moreover, the constituent cells proliferated in vitro. To investigate the interactions responsible for the experimentally observed cellular rearrangements, we built lattice models of the bioprinted constructs and simulated their evolution using Metropolis Monte Carlo methods. Although unable to replicate the complexity of the TME, the approach presented here enables the self-assembly and co-culture of several cell types of the TME. Further studies will evaluate whether the bioprinted constructs can evolve in vivo in animal models. If they become connected to the host vasculature, they may turn into a fully organized TME.
Databáze: MEDLINE
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