Oral Intake of Lumisterol Affects the Metabolism of Vitamin D.

Autor: Kotwan J; Institute of Agricultural and Nutritional Sciences, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany.; Competence Cluster for Nutrition and Cardiovascular Health (nutriCARD) Halle-Jena-Leipzig, Jena, Germany., Kühn J; Institute of Agricultural and Nutritional Sciences, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany., Baur AC; Institute of Agricultural and Nutritional Sciences, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany.; Competence Cluster for Nutrition and Cardiovascular Health (nutriCARD) Halle-Jena-Leipzig, Jena, Germany., Stangl GI; Institute of Agricultural and Nutritional Sciences, Martin Luther University Halle-Wittenberg, Halle (Saale), Germany.; Competence Cluster for Nutrition and Cardiovascular Health (nutriCARD) Halle-Jena-Leipzig, Jena, Germany.
Jazyk: angličtina
Zdroj: Molecular nutrition & food research [Mol Nutr Food Res] 2021 Jul; Vol. 65 (14), pp. e2001165. Date of Electronic Publication: 2021 Jun 18.
DOI: 10.1002/mnfr.202001165
Abstrakt: Scope: The treatment of food with ultraviolet-B (UV-B) light to increase the vitamin D content is accompanied by the formation of photoisomers, such as lumisterol 2 . The physiological impact of photoisomers is largely unknown.
Methods and Results: Three groups of C57Bl/6 mice are fed diets containing 50 µg kg -1 deuterated vitamin D 3 with 0, 50 (moderate-dose) or 2000 µg kg -1 (high-dose) lumisterol 2 for four weeks. Considerable quantities of lumisterol 2 and vitamin D 2 are found in the plasma and tissues of mice fed with 2000 µg kg -1 lumisterol 2 but not in those fed 0 or 50 µg kg -1 lumisterol 2 . Mice fed with 2000 µg kg -1 lumisterol 2 showed strongly reduced deuterated 25-hydroxyvitamin D 3 (-50%) and calcitriol (-80%) levels in plasma, accompanied by downregulated mRNA abundance of cytochrom P450 (Cyp)27b1 and upregulated Cyp24a1 in the kidneys. Increased tissue levels of vitamin D 2 were also seen in mice in a second study that are kept on a diet with 0.2% UV-B exposed yeast versus those fed 0.2% untreated yeast containing iso-amounts of vitamin D 2 .
Conclusion: High doses of lumisterol 2 can enter the body, induce the formation of vitamin D 2 , reduce the levels of 25(OH)D 3 and calcitriol and strongly impact the expression of genes involved in the degradation and synthesis of bioactive vitamin D.
(© 2021 The Authors. Molecular Nutrition & Food Research published by Wiley-VCH GmbH.)
Databáze: MEDLINE
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