Existence of SARS-CoV-2 RNA on ambient particulate matter samples: A nationwide study in Turkey.

Autor: Kayalar Ö; Koç University Research Center for Translational Medicine (KUTTAM), Istanbul, Turkey., Arı A; Department of Environmental Engineering, Faculty of Engineering, Bolu Abant Izzet Baysal University, Gölköy Campus, Bolu, Turkey., Babuççu G; Koc University Research Center for Infectious Diseases, Department of Medical Microbiology, Koç University School of Medicine, Istanbul, Turkey., Konyalılar N; Koç University Research Center for Translational Medicine (KUTTAM), Istanbul, Turkey., Doğan Ö; Koc University Research Center for Infectious Diseases, Department of Medical Microbiology, Koç University School of Medicine, Istanbul, Turkey., Can F; Koc University Research Center for Infectious Diseases, Department of Medical Microbiology, Koç University School of Medicine, Istanbul, Turkey., Şahin ÜA; Department of Environmental Engineering, Engineering Faculty, Istanbul University-Cerrahpaşa, Avcılar, Istanbul, Turkey., Gaga EO; Department of Environmental Engineering, Faculty of Engineering, Eskişehir Technical University, Eskişehir, Turkey., Levent Kuzu S; Department of Environmental Engineering, Civil Engineering Faculty, Yildiz Technical University, Esenler, Istanbul, Turkey., Arı PE; Department of Environmental Engineering, Faculty of Engineering, Bolu Abant Izzet Baysal University, Gölköy Campus, Bolu, Turkey., Odabaşı M; Department of Environmental Engineering, Dokuz Eylül University, Izmir, Turkey., Taşdemir Y; Department of Environmental Engineering, Faculty of Engineering, Bursa Uludağ University, Bursa, Turkey., Sıddık Cindoruk S; Department of Environmental Engineering, Faculty of Engineering, Bursa Uludağ University, Bursa, Turkey., Esen F; Department of Environmental Engineering, Faculty of Engineering, Bursa Uludağ University, Bursa, Turkey., Sakın E; Department of Environmental Engineering, Faculty of Engineering, Bursa Uludağ University, Bursa, Turkey., Çalışkan B; Department of Environmental Engineering, Faculty of Engineering, Bursa Uludağ University, Bursa, Turkey., Tecer LH; Department of Environmental Engineering, Çorlu Faculty of Engineering, Namık Kemal University, Tekirdağ, Turkey., Fıçıcı M; Department of Environmental Engineering, Çorlu Faculty of Engineering, Namık Kemal University, Tekirdağ, Turkey., Altın A; Department of Environmental Engineering, Zonguldak Bülent Ecevit University, Zonguldak, Turkey., Onat B; Department of Environmental Engineering, Engineering Faculty, Istanbul University-Cerrahpaşa, Avcılar, Istanbul, Turkey., Ayvaz C; Department of Environmental Engineering, Engineering Faculty, Istanbul University-Cerrahpaşa, Avcılar, Istanbul, Turkey., Uzun B; Department of Environmental Engineering, Engineering Faculty, Istanbul University-Cerrahpaşa, Avcılar, Istanbul, Turkey., Saral A; Department of Environmental Engineering, Civil Engineering Faculty, Yildiz Technical University, Esenler, Istanbul, Turkey., Döğeroğlu T; Department of Environmental Engineering, Faculty of Engineering, Eskişehir Technical University, Eskişehir, Turkey., Malkoç S; Department of Environmental Engineering, Faculty of Engineering, Eskişehir Technical University, Eskişehir, Turkey., Üzmez ÖÖ; Department of Environmental Engineering, Faculty of Engineering, Eskişehir Technical University, Eskişehir, Turkey., Kunt F; Department of Environmental Engineering, Necmettin Erbakan University, Konya, Turkey., Aydın S; Department of Environmental Engineering, Necmettin Erbakan University, Konya, Turkey., Kara M; Department of Environmental Engineering, Dokuz Eylül University, Izmir, Turkey., Yaman B; Department of Environmental Engineering, Dokuz Eylül University, Izmir, Turkey., Doğan G; Department of Environmental Engineering, Akdeniz University, Antalya, Turkey., Olgun B; Department of Environmental Engineering, Akdeniz University, Antalya, Turkey., Dokumacı EN; Department of Environmental Engineering, Akdeniz University, Antalya, Turkey., Güllü G; Department of Environmental Engineering, Hacettepe University, Ankara, Turkey., Uzunpınar ES; Department of Environmental Engineering, Middle East Technical University, Ankara, Turkey., Bayram H; Koç University Research Center for Translational Medicine (KUTTAM), Istanbul, Turkey; Department of Pulmonary Medicine, School of Medicine, Koç University, Istanbul, Turkey. Electronic address: habayram@ku.edu.tr.
Jazyk: angličtina
Zdroj: The Science of the total environment [Sci Total Environ] 2021 Oct 01; Vol. 789, pp. 147976. Date of Electronic Publication: 2021 May 25.
DOI: 10.1016/j.scitotenv.2021.147976
Abstrakt: Coronavirus disease 2019 (COVID-19) is caused by the SARS-CoV-2 virus and has been affecting the world since the end of 2019. The disease led to significant mortality and morbidity in Turkey, since the first case was reported on March 11th, 2020. Studies suggest a positive association between air pollution and SARS-CoV-2 infection. The aim of the present study was to investigate the role of ambient particulate matters (PM), as potential carriers for SARS-CoV-2. Ambient PM samples in various size ranges were collected from 13 sites including urban and urban-background locations and hospital gardens in 10 cities across Turkey between 13th of May and 14th of June 2020 to investigate the possible presence of SARS-CoV-2 RNA on ambient PM. A total of 203 daily samples (TSP, n = 80; PM 2.5 , n = 33; PM 2.5 - 10 , n = 23; PM 10 μm, n = 19; and 6 size segregated PM, n = 48) were collected using various samplers. The N1 gene and RdRP gene expressions were analyzed for the presence of SARS-CoV-2, as suggested by the Centers for Disease Control and Prevention (CDC). According to real time (RT)-PCR and three-dimensional (3D) digital (d) PCR analysis, dual RdRP and N1 gene positivity were detected in 20 (9.8%) samples. Ambient PM-bound SARS-CoV-2 was analyzed quantitatively and the air concentrations of the virus ranged from 0.1 copies/m 3 to 23 copies/m 3 . The highest percentages of virus detection on PM samples were from hospital gardens in Tekirdağ, Zonguldak, and Istanbul, especially in PM 2.5 mode. Findings of this study have suggested that SARS-CoV-2 may be transported by ambient particles, especially at sites close to the infection hot-spots. However, whether this has an impact on the spread of the virus infection remains to be determined.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2021 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: