Mass spectral analysis of secondary metabolites from Zingiber montanum rhizome extract using UHPLC-HR-ESI-QTOF-MS/MS.

Autor:	Kabkrathok P; Department of Pharmacognosy and Center of Innovative Pharmacy for Pharmaceutical and Herbal Product Development, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand., Jarussophon S; Nano Agricultural Chemistry and Processing Research Team, National Nanotechnology Center, National Science and Technology Development Agency, Pathum Thani, Thailand., Unger O; Nano Characterization Laboratory, National Nanotechnology Center, National Science and Technology Development Agency, Pathum Thani, Thailand., Lomarat P; Department of Food Chemistry, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand., Reutrakul V; Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Mahidol University, Bangkok, Thailand., Pittayanurak P; Department of Chemistry and Center of Excellence for Innovation in Chemistry, Faculty of Science, Mahidol University, Bangkok, Thailand., Bongcheewin B; Department of Pharmaceutical Botany, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand., Anantachoke N; Department of Pharmacognosy and Center of Innovative Pharmacy for Pharmaceutical and Herbal Product Development, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand.
Jazyk:	angličtina
Zdroj:	Phytochemical analysis : PCA [Phytochem Anal] 2022 Jan; Vol. 33 (1), pp. 57-71. Date of Electronic Publication: 2021 May 30.
DOI:	10.1002/pca.3068
Abstrakt:	Introduction: Zingiber montanum (J.Koenig) Link ex A.Dietr. is a popular medicinal plant in Thailand. Its rhizomes have been used as an ingredient in various Thai traditional medicine formulas. While many reports have focused on the chemical constituents and biological activities of this plant, a comprehensive study on secondary metabolite profiling using tandem mass spectrometry has, to this point, never been documented. Objective: To analyze the chemical constituents in Z. montanum rhizomes using ultra-high performance liquid chromatography coupled with ultra-high-resolution electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UHPLC-HR-ESI-QTOF-MS/MS) analyses and to utilize the characteristic fragmentation patterns of these compounds to facilitate their identification. Methodology: UHPLC-HR-ESI-QTOF-MS/MS in positive ion mode was used for chemical identification of secondary metabolites from the ethanolic extract of the plant material. MS/MS data of some known reference compounds, together with detailed fragmentation pattern information of several compounds obtained from the crude extract, were used to elucidate their chemical structures. Results: In this work, one benzaldehyde, ten phenylbutenoid monomers, six curcuminoids, and nine phenylbutenoid dimers were assigned based on their characteristic fragment ions. Among these compounds, 2-(3,4-dimethoxystyryl)oxirane was tentatively suggested as a potential new compound. Several characteristic fragment ions from these compounds were assigned and the relative ion abundance of these was also used to differentiate the chemical structures of compounds having the same molecular mass. Conclusions: The results will benefit future high-throughput screening of bioactive compounds and method development for the quality control of raw materials and herbal drugs derived from Z. montanum rhizome extracts. (© 2021 John Wiley & Sons, Ltd.)
Databáze:	MEDLINE
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