Effects of Long-Term Methotrexate, Infliximab, and Tocilizumab Administration on Bone Microarchitecture and Tendon Morphology in Healthy Wistar Rats.

Autor:	Poutoglidou F; Department of Clinical Pharmacology, Aristotle University of Thessaloniki, Thessaloniki, GRC., Pourzitaki C; Department of Clinical Pharmacology, Aristotle University of Thessaloniki, Thessaloniki, GRC., Manthou ME; Laboratory of Histology and Embryology, Aristotle University of Thessaloniki, Thessaloniki, GRC., Samoladas E; Division of Orthopaedics, Genimatas Hospital, Aristotle University of Thessaloniki, Thessaloniki, GRC., Malliou F; Department of Clinical Pharmacology, Aristotle University of Thessaloniki, Thessaloniki, GRC., Saitis A; Department of Clinical Pharmacology, Aristotle University of Thessaloniki, Thessaloniki, GRC., Kouvelas D; Department of Clinical Pharmacology, Aristotle University of Thessaloniki, Thessaloniki, GRC.
Jazyk:	angličtina
Zdroj:	Cureus [Cureus] 2021 Apr 26; Vol. 13 (4), pp. e14696. Date of Electronic Publication: 2021 Apr 26.
DOI:	10.7759/cureus.14696
Abstrakt:	Objective Rheumatic diseases are associated with bone loss, both systemic and periarticular, and tendon abnormalities. The aim of this study is to examine the effect of three antiarthritic drugs, methotrexate, an anti-folate metabolite; infliximab, a Tumor Necrosis Factor-alpha (TNF-α) inhibitor; and tocilizumab, an antibody against Interleukin-6 (IL-6) receptor, on bone microarchitecture and tendon morphology in the absence of an inflammatory state. Materials and methods Thirty-five, 8- to 9-week-old, male, Wistar rats were randomly allocated into five groups: negative control (CTRL), vehicle (VEH), methotrexate (MTX), infliximab (INFX), and tocilizumab (TCZ). After 8 weeks of antiarthritic drug intraperitoneal administration, animals were euthanized and rat tibiae and patellar tendons were histologically examined. Results All sections exhibited normal bone microarchitecture. Histological scores in all groups corresponded to normal bone mineral density. No no apparent differences in tenocyte morphology and architecture of collagen fibers were observed. Conclusions The results of this study indicate that long-term administration of methotrexate, infliximab, and tocilizumab had no effect on bone microarchitecture and tendon morphology in rats in the absence of an inflammatory condition. Competing Interests: The authors have declared that no competing interests exist. (Copyright © 2021, Poutoglidou et al.)
Databáze:	MEDLINE
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