| Autor: |
Rich TC; Pharmacology, University of South Alabama, AL 36688.; Center for Lung Biology, University of South Alabama, AL 36688.; Basic Medical Sciences Graduate Program, University of South Alabama, AL 36688., Annamdevula N; Center for Lung Biology, University of South Alabama, AL 36688.; Basic Medical Sciences Graduate Program, University of South Alabama, AL 36688., Trinh K; Chemical and Biomolecular Engineering, University of South Alabama, AL 36688., Britain AL; Pharmacology, University of South Alabama, AL 36688.; Center for Lung Biology, University of South Alabama, AL 36688., Mayes SA; Chemical and Biomolecular Engineering, University of South Alabama, AL 36688., Griswold JR; Chemical and Biomolecular Engineering, University of South Alabama, AL 36688., Deal J; Center for Lung Biology, University of South Alabama, AL 36688.; Basic Medical Sciences Graduate Program, University of South Alabama, AL 36688., Hoffman C; Pharmacology, University of South Alabama, AL 36688., West S; Biomedical Sciences, University of South Alabama, AL 36688., Leavesley SJ; Pharmacology, University of South Alabama, AL 36688.; Center for Lung Biology, University of South Alabama, AL 36688.; Basic Medical Sciences Graduate Program, University of South Alabama, AL 36688.; Chemical and Biomolecular Engineering, University of South Alabama, AL 36688. |
| Abstrakt: |
Cyclic AMP (cAMP) is a ubiquitous second messenger known to differentially regulate many cellular functions. Several lines of evidence suggest that the distribution of cAMP within cells is not uniform. However, to date, no studies have measured the kinetics of 3D cAMP distributions within cells. This is largely due to the low signal-to-noise ratio of FRET-based probes. We previously reported that hyperspectral imaging improves the signal-to-noise ratio of FRET measurements. Here we utilized hyperspectral imaging approaches to measure FRET signals in five dimensions (5D) - three spatial (x, y, z), wavelength (λ), and time (t) - allowing us to visualize cAMP gradients in pulmonary endothelial cells. cAMP levels were measured using a FRET-based sensor (H188) comprised of a cAMP binding domain sandwiched between FRET donor and acceptor - Turquoise and Venus fluorescent proteins. We observed cAMP gradients in response to 0.1 or 1 μM isoproterenol, 0.1 or 1 μM PGE 1 , or 50 μM forskolin. Forskolin- and isoproterenol-induced cAMP gradients formed from the apical (high cAMP) to basolateral (low cAMP) face of cells. In contrast, PGE 1 -induced cAMP gradients originated from both the basolateral and apical faces of cells. Data suggest that 2D (x,y) studies of cAMP compartmentalization may lead to erroneous conclusions about the existence of cAMP gradients, and that 3D (x,y,z) studies are required to assess mechanisms of signaling specificity. Results demonstrate that 5D imaging technologies are powerful tools for measuring biochemical processes in discrete subcellular domains. This work was supported by NIH P01HL066299, R01HL058506, S10RR027535, AHA 16PRE27130004 and the Abraham Mitchell Cancer Research Fund. |
