Chemical composition and potentiating action of Norfloxacin mediated by the essential oil of Piper caldense C.D.C. against Staphylococcus aureus strains overexpressing efflux pump genes.

Autor: Leal ALAB; Department of Biological Chemistry, Regional University of Cariri, Crato, CE, Brazil.; Department of Microbiology and Parasitolog, Federal University of Piaui, Teresina, PI, Brazil., Bezerra CF; Department of Pharmaceutical Sciences, Federal University of Pernambuco, Recife, PE, Brazil., Confortin C; Postgraduate Programme in Sustainable Territorial, Federal University of Paraná, Curitiba, PR, Brazil., da Silva LE; Postgraduate Programme in Sustainable Territorial, Federal University of Paraná, Curitiba, PR, Brazil., Marinho EM; Department of Analytical Chemistry and Physical Chemistry, Federal University of Ceará, Fortaleza, CE, Brazil., Marinho MM; Faculty of Education, Science and Letters of Iguatu, State University of Ceará, Iguatu, CE, Brazil., Vasconcelos MA; Minas Gerais State University, Divinópolis Unit, MG, Brazil., da Silva TG; Department of Antibiotics, Drug Planning and Synthesis Laboratory-LPSF, Federal University of Pernambuco, Cidade Universitária, Recife, PE, Brazil., Marinho ES; Group of Theoretical Chemistry and Electrochemistry, State University of Ceará, Campus FAFIDAM, Limoeiro do Norte, Fortaleza, CE, Brazil., Teixeira AMR; Department of Biological Chemistry, Regional University of Cariri, Crato, CE, Brazil., Coutinho HDM; Department of Biological Chemistry, Regional University of Cariri, Crato, CE, Brazil., Barreto HM; Department of Microbiology and Parasitolog, Federal University of Piaui, Teresina, PI, Brazil., Dos Santos HS; Department of Biological Chemistry, Regional University of Cariri, Crato, CE, Brazil. helciodossantos@gmail.com.; Center for Exact Sciences and Technology, Vale do Acarau State University, Sobral, CE, Brazil. helciodossantos@gmail.com.
Jazyk: angličtina
Zdroj: Archives of microbiology [Arch Microbiol] 2021 Sep; Vol. 203 (7), pp. 4727-4736. Date of Electronic Publication: 2021 May 29.
DOI: 10.1007/s00203-021-02393-5
Abstrakt: Infectious diseases caused by multidrug-resistant microorganisms has increased in the last years. Piper species have been reported as a natural source of phytochemicals that can help in combating fungal and bacterial infections. This study had as objectives characterize the chemical composition of the essential oil from Piper caldense (EOPC), evaluate its potential antimicrobial activity, and investigate the synergistic effect with Norfloxacin against multidrug-resistant S. aureus overproducing efflux pumps, as well as, verify the EOPC ability to inhibit the Candida albicans filamentation. EOPC was extracted by hydrodistillation, and the chemical constituents were identified by gas chromatography, allowing the identification of 24 compounds (91.9%) classified as hydrocarbon sesquiterpenes (49.6%) and oxygenated sesquiterpenes (39.5%). Antimicrobial tests were performed using a 96-well plate microdilution method against C. albicans ATCC 10231, Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923 standard strains, as well as against multidrug-resistant strains S. aureus SA1199B (overexpressing norA gene), S. aureus K2068 (overexpressing mepA gene) and S. aureus K4100 (overexpressing qacC gene). The oil showed activity against C. albicans ATCC 10231 (≥ 512 µg/mL) and was able to inhibit hyphae formation, an important mechanism of virulence of C. albicans. On the other hand, EOPC was inactive against all bacterial strains tested (≤ 1,024 µg mL). However, when combined with Norfloxacin at subinhibitory concentration EOPC reduced the Norfloxacin and Ethidium bromide MIC values against S. aureus strains SA1199B, K2068 and K4100. These results indicate that EOPC is a source of phytochemicals acting as NorA, MepA and QacC inhibitors.
(© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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