DROP: Molecular voucher database for identification of Drosophila parasitoids.

Autor: Lue CH; Biology Centre of the Czech Academy of Sciences, Institute of Entomology, Ceske Budejovice, Czech Republic.; Department of Biology, Brooklyn College, City University of New York (CUNY), Brooklyn, NY, USA., Buffington ML; Systematic Entomology Laboratory, ARS/USDA c/o Smithsonian Institution, National Museum of Natural History, Washington, DC, USA., Scheffer S; Systematic Entomology Laboratory, ARS/USDA c/o Smithsonian Institution, National Museum of Natural History, Washington, DC, USA., Lewis M; Systematic Entomology Laboratory, ARS/USDA c/o Smithsonian Institution, National Museum of Natural History, Washington, DC, USA., Elliott TA; Centre for Biodiversity Genomics, University of Guelph, Guelph, ON, Canada., Lindsey ARI; Department of Entomology, University of Minnesota, Saint Paul, MN, USA., Driskell A; Laboratories of Analytical Biology, Smithsonian Institution, National Museum of Natural History, Washington, DC, USA., Jandova A; Biology Centre of the Czech Academy of Sciences, Institute of Entomology, Ceske Budejovice, Czech Republic., Kimura MT; Hokkaido University Museum, Sapporo, Hokkaido, Japan., Carton Y; 'Évolution, Génomes, Comportement, Écologie', CNRS et Université Paris-Saclay, Paris, France., Kula RR; Systematic Entomology Laboratory, ARS/USDA c/o Smithsonian Institution, National Museum of Natural History, Washington, DC, USA., Schlenke TA; Department of Entomology, University of Arizona, Tucson, AZ, USA., Mateos M; Wildlife and Fisheries Sciences Department, Texas A&M University, College Station, TX, USA., Govind S; The Graduate Center of the City University of New York, New York, NY, USA., Varaldi J; CNRS, Laboratoire de Biométrie et Biologie Evolutive, UMR 5558, Université de Lyon, Université Lyon 1, Villeurbanne, France., Guerrieri E; CNR-Institute for Sustainable Plant Protection (CNR-IPSP), National Research Council of Italy, Portici, Italy., Giorgini M; CNR-Institute for Sustainable Plant Protection (CNR-IPSP), National Research Council of Italy, Portici, Italy., Wang X; United States Department of Agriculture, Agricultural Research Services, Beneficial Insects Introduction Research Unit, Newark, DE, USA., Hoelmer K; United States Department of Agriculture, Agricultural Research Services, Beneficial Insects Introduction Research Unit, Newark, DE, USA., Daane KM; Department of Environmental Science, Policy and Management, University of California, Berkeley, CA, USA., Abram PK; Agriculture and Agri-Food Canada, Agassiz Research and Development Centre, Agassiz, BC, Canada., Pardikes NA; Biology Centre of the Czech Academy of Sciences, Institute of Entomology, Ceske Budejovice, Czech Republic., Brown JJ; Biology Centre of the Czech Academy of Sciences, Institute of Entomology, Ceske Budejovice, Czech Republic.; Faculty of Science, University of South Bohemia, Branisovska 31, Czech Republic., Thierry M; Biology Centre of the Czech Academy of Sciences, Institute of Entomology, Ceske Budejovice, Czech Republic.; Faculty of Science, University of South Bohemia, Branisovska 31, Czech Republic., Poirié M; INRAE, CNRS. and Evolution and Specificity of Multitrophic Interactions (ESIM) Sophia Agrobiotech Institute, Université 'Côte d'Azur', Sophia Antipolis, France., Goldstein P; Systematic Entomology Laboratory, ARS/USDA c/o Smithsonian Institution, National Museum of Natural History, Washington, DC, USA., Miller SE; Smithsonian Institution, National Museum of Natural History, Washington, DC, USA., Tracey WD; Department of Biology, Indiana University Bloomington, Bloomington, IN, USA.; Gill Center for Biomolecular Science, Indiana University Bloomington, Bloomington, IN, USA., Davis JS; Department of Biology, Indiana University Bloomington, Bloomington, IN, USA.; Biology Department, University of Kentucky, Lexington, KY, USA., Jiggins FM; Department of Genetics, University of Cambridge, Cambridge, UK., Wertheim B; Groningen Institute for Evolutionary Life Sciences, University of Groningen, Groningen, the Netherlands., Lewis OT; Department of Zoology, University of Oxford, Oxford, UK., Leips J; Department of Biological Sciences, University of Maryland Baltimore County, Baltimore, MD, USA., Staniczenko PPA; Department of Biology, Brooklyn College, City University of New York (CUNY), Brooklyn, NY, USA., Hrcek J; Biology Centre of the Czech Academy of Sciences, Institute of Entomology, Ceske Budejovice, Czech Republic.; Faculty of Science, University of South Bohemia, Branisovska 31, Czech Republic.
Jazyk: angličtina
Zdroj: Molecular ecology resources [Mol Ecol Resour] 2021 Oct; Vol. 21 (7), pp. 2437-2454. Date of Electronic Publication: 2021 Jun 10.
DOI: 10.1111/1755-0998.13435
Abstrakt: Molecular identification is increasingly used to speed up biodiversity surveys and laboratory experiments. However, many groups of organisms cannot be reliably identified using standard databases such as GenBank or BOLD due to lack of sequenced voucher specimens identified by experts. Sometimes a large number of sequences are available, but with too many errors to allow identification. Here, we address this problem for parasitoids of Drosophila by introducing a curated open-access molecular reference database, DROP (Drosophila parasitoids). Identifying Drosophila parasitoids is challenging and poses a major impediment to realize the full potential of this model system in studies ranging from molecular mechanisms to food webs, and in biological control of Drosophila suzukii. In DROP, genetic data are linked to voucher specimens and, where possible, the voucher specimens are identified by taxonomists and vetted through direct comparison with primary type material. To initiate DROP, we curated 154 laboratory strains, 856 vouchers, 554 DNA sequences, 16 genomes, 14 transcriptomes, and six proteomes drawn from a total of 183 operational taxonomic units (OTUs): 114 described Drosophila parasitoid species and 69 provisional species. We found species richness of Drosophila parasitoids to be heavily underestimated and provide an updated taxonomic catalogue for the community. DROP offers accurate molecular identification and improves cross-referencing between individual studies that we hope will catalyse research on this diverse and fascinating model system. Our effort should also serve as an example for researchers facing similar molecular identification problems in other groups of organisms.
(© 2021 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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