Detection of respiratory viruses in primary cholesteatoma tissues.

Autor: Viana RMM; Department of Ophthalmology, Otorhinolaryngology, and Head and Neck Surgery, Ribeirão Preto School of Medicine, University of São Paulo (USP), Ribeirão Preto, São Paulo, Brazil.; Department of Molecular and Cell Biology, Ribeirão Preto School of Medicine, University of São Paulo (USP), Ribeirão Preto, São Paulo, Brazil., Souza JP; Department of Molecular and Cell Biology, Ribeirão Preto School of Medicine, University of São Paulo (USP), Ribeirão Preto, São Paulo, Brazil., Jorge DMM; Department of Molecular and Cell Biology, Ribeirão Preto School of Medicine, University of São Paulo (USP), Ribeirão Preto, São Paulo, Brazil., Martins RB; Department of Molecular and Cell Biology, Ribeirão Preto School of Medicine, University of São Paulo (USP), Ribeirão Preto, São Paulo, Brazil., Castro IA; Department of Molecular and Cell Biology, Ribeirão Preto School of Medicine, University of São Paulo (USP), Ribeirão Preto, São Paulo, Brazil., Cardoso RS; Department of Molecular and Cell Biology, Ribeirão Preto School of Medicine, University of São Paulo (USP), Ribeirão Preto, São Paulo, Brazil., Volpini LPB; Post-Graduate Program in infectious Diseases, Federal University of Espírito Santo, Vitória, Brazil., de Souza Luna LK; Medicine Department, Clinical Virology Laboratory, Infectious Diseases Unit, São Paulo Federal University, São Paulo, Brazil., Spano LC; Post-Graduate Program in infectious Diseases, Federal University of Espírito Santo, Vitória, Brazil.; Departament of Pathology, Center of Health Sciences, Federal University of Espírito Santo, Vitória, Brazil., Bellei NCJ; Medicine Department, Clinical Virology Laboratory, Infectious Diseases Unit, São Paulo Federal University, São Paulo, Brazil., Chahud F; Department of Pathology and Forensic Medicine, Ribeirão Preto School of Medicine, University of São Paulo (USP), São Paulo, Brazil., Arruda E; Department of Molecular and Cell Biology, Ribeirão Preto School of Medicine, University of São Paulo (USP), Ribeirão Preto, São Paulo, Brazil., A Hyppolito M; Department of Ophthalmology, Otorhinolaryngology, and Head and Neck Surgery, Ribeirão Preto School of Medicine, University of São Paulo (USP), Ribeirão Preto, São Paulo, Brazil.
Jazyk: angličtina
Zdroj: Journal of medical virology [J Med Virol] 2021 Nov; Vol. 93 (11), pp. 6132-6139. Date of Electronic Publication: 2021 Jun 10.
DOI: 10.1002/jmv.27107
Abstrakt: Cholesteatomas are frequent middle ear benign tumors of unknown etiology. Infectious agents have been considered as possible contributing factors in the pathogenesis of cholesteatomas. Aiming to investigate the presence of respiratory viruses in primary cholesteatoma tissues, 26 formalin-fixed paraffin-embedded primary cholesteatoma tissues obtained from patients seen at the of the Clinical Hospital of the University of São Paulo School of Medicine, in Ribeirão Preto, Brazil were tested by real-time polymerase chain reaction (PCR). Considering the PCR results, 35% of the tissues were positive for human rhinovirus (HRV), 15.3% for human enterovirus (EV), 3.8% for human metapneumovirus (HMPV), and 3.8% for human bocavirus (HBoV). Serial immunohistochemistry for virus antigens and cell surface markers evidenced that the viruses were associated with fibroblasts, dendritic cells, macrophages, B lymphocytes, CD4 + , and CD8 + T lymphocytes. These findings indicate for the first time the presence of active respiratory virus infection in primary cholesteatoma tissues, suggesting that persisting virus infection in the middle could play a role in the pathogenesis and evolution of cholesteatomas.
(© 2021 Wiley Periodicals LLC.)
Databáze: MEDLINE
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