Effects of infrared light laser therapy on in vivo and in vitro periodontitis models.

Autor: da Cruz Galhardo Camargo GA; Institute of Science and Technology, Federal University of São Paulo, São José dos Campos, São Paulo, Brazil.; Post-Graduation Program in Dentistry, Health Institute of Nova Friburgo, PPGO-ISNF, Specific Formation Department, Fluminense Federal University, Nova Friburgo, Rio de Janeiro, Brazil., de Oliveira Barbosa LM; Department of Basic Sciences, Federal Fluminense University, Nova Friburgo, Rio de Janeiro, Brazil., Stumbo MB; Department of Basic Sciences, Federal Fluminense University, Nova Friburgo, Rio de Janeiro, Brazil., Thurler Júnior JC; Department of Basic Sciences, Federal Fluminense University, Nova Friburgo, Rio de Janeiro, Brazil., da Costa GRM; Department of Basic Sciences, Federal Fluminense University, Nova Friburgo, Rio de Janeiro, Brazil., Domingos-Vieira AC; Department of Oral Pathology, Oral Radiology, and Oral Diagnosis, School of Dentistry, Federal University of Rio de Janeiro, Nova Friburgo, Rio de Janeiro, Brazil., Pascoal VDB; Post Graduation Program in Science and Biotechnology, Biology Institute, Fluminense Federal University, Niterói, Rio de Janeiro, Brazil., Robbs BK; Postgraduate Program in Applied Science for Health Products, Faculty of Pharmacy, Fluminense Federal University, Niterói, Rio de Janeiro, Brazil.; Postgraduate Program in Dentistry, Health Institute of Nova Friburgo, Fluminense Federal University, Nova Friburgo, Rio de Janeiro, Brazil., Lopes RT; Nuclear Engineering Program (PEN/COPPE), Federal University of Rio de Janeiro (UFRJ), Nova Friburgo, Rio de Janeiro, Brazil., de Araujo OMO; Nuclear Engineering Program (PEN/COPPE), Federal University of Rio de Janeiro (UFRJ), Nova Friburgo, Rio de Janeiro, Brazil., Capelo LP; Institute of Science and Technology, Federal University of São Paulo, São José dos Campos, São Paulo, Brazil.
Jazyk: angličtina
Zdroj: Journal of periodontology [J Periodontol] 2022 Feb; Vol. 93 (2), pp. 308-319. Date of Electronic Publication: 2021 Jun 19.
DOI: 10.1002/JPER.20-0842
Abstrakt: Background: This study evaluated the effects of infrared light laser therapy (ILLT) on ligature-induced periodontitis in rats using micro-computed tomography (micro-CT), histology, fibroblast migration, and viability analysis.
Methods: Forty-eight rats were randomly distributed into three groups: control (no periodontitis), PDC (periodontitis without laser therapy), and PD+L (periodontitis with laser therapy). Periodontitis was induced by ligature placement for 4 weeks. The 12-week-old rats (baseline) were subjected to laser treatment and euthanized 30 days after. After treatment, the mandibular first molars were prepared for micro-CT scanning, and histological sections were assessed as to the cementoenamel junction, alveolar bone crest, and polymorphonuclear (PMN) cell infiltration. In vitro assays were carried out to examine NIH/3T3 fibroblast viability after laser therapy.
Results: Migration and cell viability assays revealed that the ILLT maintained fibroblast cell viability with 4 J/cm 2 , reaching 100% healing. The control group (at baseline and 30 days) presented a statistically significant difference from the PDC group at 30 days in terms of distance from the cementoenamel junction to the alveolar bone crest (CEJ-ABC). The PD+L group showed a statistically substantial difference from the PDC group at 30 days in terms of trabecular thickness (Tb.Th), degree of anisotropy (DA), and closed porosity percentage (Po%).
Conclusion: ILLT seemed to preserve the bone structure in the in vivo periodontitis induction model at 30 days and did not reduce cell viability or increase fibroblast migration in vitro. The ILLT provides positive effects on mandibular bone microstructure.
(© 2021 American Academy of Periodontology.)
Databáze: MEDLINE
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