| Autor: |
Sahoo PR; Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Sendai, Miyagi, Japan., Kowada T; Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Sendai, Miyagi, Japan., Mizukami S; Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Sendai, Miyagi, Japan. shin.mizukami@tohoku.ac.jp. |
| Jazyk: |
angličtina |
| Zdroj: |
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2274, pp. 237-243. |
| DOI: |
10.1007/978-1-0716-1258-3_20 |
| Abstrakt: |
Living cells dynamically change their morphology and function according to the cell cycle. Long-term observations of living cells are privileged when we spy the unique, cell cycle-driven molecular events, which cannot be obtained from short-term ones. Mg 2+ , a metal ion abundant in cells, has been shown to be involved in a variety of physiological phenomena by noninvasive cellular observation using fluorescence microscopy. However, long-term observation of Mg 2+ in cells has been a great challenge. Herein, we present a protocol for the long-term microscopic imaging of intracellular Mg 2+ levels using a small molecule-protein hybrid fluorescent probe we developed. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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