Thyroid Hormone Deficiency Modifies Hepatic Lipid Droplet Morphology and Molecular Properties in Lithogenic-Diet Supplemented Mice.

Autor:	Kube I; Department of Endocrinology, Diabetes and Metabolism and Clinical Chemistry - Division of Laboratory Research, University of Duisburg-Essen, Germany., Jastrow H; Faculty of Medicine, University of Duisburg-Essen - Institute of Anatomy and Imaging Center Essen, Germany., Führer D; Department of Endocrinology, Diabetes and Metabolism and Clinical Chemistry - Division of Laboratory Research, University of Duisburg-Essen, Germany., Zwanziger D; Department of Endocrinology, Diabetes and Metabolism and Clinical Chemistry - Division of Laboratory Research, University of Duisburg-Essen, Germany.
Jazyk:	angličtina
Zdroj:	Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Association [Exp Clin Endocrinol Diabetes] 2021 Dec; Vol. 129 (12), pp. 926-930. Date of Electronic Publication: 2021 May 28.
DOI:	10.1055/a-1404-7939
Abstrakt:	Objective: Thyroid hormones have been associated with a hepatic lipid lowering effect and thyroid function has been shown to play a substantial role in development of non-alcoholic fatty liver disease. Hepatic lipid droplets differ in the number, size and molecular properties depending on metabolic state or pathological condition. However, in how far thyroid hormone deficiency affects hepatic lipid droplet morphology and molecular properties is still poorly understood. Therefore, we performed a study in mice using a lithogenic diet model of steatohepatitis and modulated the thyroid hormone status. Methods: Male and female three months old C57BL/6 mice were divided into a euthyroid (control), a lithogenic (litho) and a lithogenic+thyroid hormone deficient (litho+hypo) group and treated for six weeks. Hepatic transmission electron microscopy and gene expression analysis of lipid-droplet associated proteins were performed. Results: Increased mean diameters of hepatic lipid droplets and a shift towards raised electron-density in lipid droplets was observed under thyroid hormone deficiency. Furthermore thyroid hormone deficiency altered hepatic expression of genes involved in lipophagy and triacylglycerol mobilization. Interestingly, while the impact of thyroid hormone deficiency on lipid droplet morphology seems to be sex-independent, hepatic lipid droplet-associated gene expression differed significantly between both sexes. Conclusion: This study demonstrates that thyroid hormone deficiency alters hepatic lipid droplet morphology and hepatic gene expression of lipid droplet-associated proteins in a lithogenic diet mouse model of steatohepatitis. Competing Interests: The authors declare that they have no conflict of interest. (Thieme. All rights reserved.)
Databáze:	MEDLINE
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