Effects of Moringa Extract on Aminoglycoside-Induced Hair Cell Death and Organ of Corti Damage.

Autor:	Broderick MT; University of Michigan Medical School, Ann Arbor, Michigan., Prince ADP; University of Michigan Medical School, Ann Arbor, Michigan., Dhukhwa A; Department of Pharmacology, School of Medicine, Southern Illinois University., Mukherjea D; Department of Otolaryngology-Head and Neck Surgery and Department of Pharmacology, Southern Illinois University Schools of Medicine, Springfield, Illinois., Jiang P; Kresge Hearing Research Institute, Department of Otolaryngology-Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan., Campbell KCM; Department of Otolaryngology-Head and Neck Surgery and Department of Pharmacology, Southern Illinois University Schools of Medicine, Springfield, Illinois., Rybak LP; Department of Medical Microbiology, Immunology, and Cell Biology, and Department of Pharmacology, Southern Illinois University Schools of Medicine, Springfield, Illinois., Brenner MJ; Kresge Hearing Research Institute, Department of Otolaryngology-Head and Neck Surgery, University of Michigan, Ann Arbor, Michigan.
Jazyk:	angličtina
Zdroj:	Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology [Otol Neurotol] 2021 Sep 01; Vol. 42 (8), pp. 1261-1268.
DOI:	10.1097/MAO.0000000000003193
Abstrakt:	Hypothesis: Moringa extract, a naturally occurring anti-oxidant, protects against aminoglycoside-induced hair cell death and hearing loss within the organ of Corti. Background: Reactive oxygen species (ROS) arise primarily in the mitochondria and have been implicated in aminoglycoside-induced ototoxicity. Mitochondrial dysfunction results in loss of membrane potential, release of caspases, and cell apoptosis. Moringa extract has not previously been examined as a protective agent for aminoglycoside-induced ototoxicity. Methods: Putative otoprotective effects of moringa extract were investigated in an organotypic model using murine organ of Corti explants subjected to gentamicin-induced ototoxicity. Assays evaluated hair cell loss, cytochrome oxidase expression, mitochondrial membrane potential integrity, and caspase activity. Results: In vitro application of moringa conferred significant protection from gentamicin-induced hair cell loss at dosages from 25 to 300 μg/mL, with dosages above 100 μg/mL conferring near complete protection. Assays demonstrated moringa extract suppression of ROS, preservation of cytochrome oxidase activity, and reduction in caspase production. Conclusion: Moringa extract demonstrated potent antioxidant properties with significant protection against gentamicin ototoxicity in cochlear explants. Competing Interests: The authors disclose no conflicts of interest. (Copyright © 2021, Otology & Neurotology, Inc.)
Databáze:	MEDLINE
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