| Autor: |
Cao T; Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Special Function Materials and Structure Design (MOE), and College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, P. R. China. qinww@lzu.edu.cn., Zhang L; Department of Radiology, Xinqiao Hospital, Army Medical University, Chongqing, 400037, P. R. China. yunliu@tmmu.edu.cn., Zheng L; Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Special Function Materials and Structure Design (MOE), and College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, P. R. China. qinww@lzu.edu.cn., Qian J; Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Special Function Materials and Structure Design (MOE), and College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, P. R. China. qinww@lzu.edu.cn., Iqbal A; Department of Chemistry, University of Balochistan, Quetta 87300, Pakistan., Iqbal K; Department of Chemistry, Sardar Bahadur Khan Women's University, Quetta 87300, Pakistan., Qin W; Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, State Key Laboratory of Applied Organic Chemistry, Key Laboratory of Special Function Materials and Structure Design (MOE), and College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, P. R. China. qinww@lzu.edu.cn., Liu Y; Department of Radiology, Xinqiao Hospital, Army Medical University, Chongqing, 400037, P. R. China. yunliu@tmmu.edu.cn. |
| Abstrakt: |
Pyroglutamate aminopeptidase-1 (PGP-1) is an important enzyme that plays an indispensable role in the process of inflammation. Up to now, few reports have been reported on the detection of PGP-1 activity in vivo and in vitro, and there are no reports on ratiometric detection. Here, the first red-emitting ratiometric fluorescent sensor (DP-1) for the specific detection of PGP-1 both in vivo and in vitro was designed and synthesized by using DCD-NH2 as the luminescent parent and pyroglutamate as a recognition group. After interacting with PGP-1, the amide bond is hydrolyzed by the enzyme and the color of the solution changes from yellow (λabs = 420 nm) to red (λabs = 520 nm), accompanied by obvious fluorescence emission wavelength change (from ∼564 nm to ∼616 nm). The probe has high specificity and sensitivity towards PGP-1 in about 10 min, and the DL is as low as 0.25 ng mL-1. Interestingly, under the stimulation of Freund's incomplete adjuvant and lipopolysaccharide, the imaging of DP-1 in HepG2 and RAW264 cells shows that the expression of PGP-1 is associated with inflammation. What's more, for the first, the imaging of a mouse tumor model confirms that the enzyme is closely related to the occurrence of some inflammation and tumor diseases. These results indicate that DP-1 can be used as an effective tool for real-time monitoring of PGP-1 levels both in vivo and in vitro and the study of inflammatory tumor pathology. |
