Molecular characterization and phylogenetic analysis of orf virus isolated from goats in Sokoto metropolis, Nigeria.

Autor:	Lawal N; Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto, Nigeria.; Central Veterinary Research Laboratory, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto, Nigeria., Ibrahim M; Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto, Nigeria., Onawala DA; Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto, Nigeria., Bello MB; Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto, Nigeria.; Central Veterinary Research Laboratory, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto, Nigeria.; Center for Advance Medical Research and Training, Usmanu Danfodiyo University, Sokoto, Nigeria., Aliyu RM; Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto, Nigeria., Baraya YS; Department of Veterinary Pathology, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto, Nigeria., Aliyu A; Department of Veterinary Pathology, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto, Nigeria., Ibrahim AM; Department of Veterinary Public Health & Preventive Medicine, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, Sokoto, Nigeria., Sa'adu A; Department of Animal Health & Production Technology, College of Agriculture & Animal Science, Wurno, Sokoto, Nigeria.
Jazyk:	angličtina
Zdroj:	Future science OA [Future Sci OA] 2021 Apr 20; Vol. 7 (6), pp. FSO700. Date of Electronic Publication: 2021 Apr 20.
DOI:	10.2144/fsoa-2020-0162
Abstrakt:	Aim: The aim of this study was to molecularly characterize orf virus isolated from clinical infections in goats in Sokoto metropolis. Materials & Methods: Embryonated chicken eggs were used to isolate orf virus according to the established protocol. Viral DNA was extracted and full coding region of B2L gene was amplified by polymerase chain reaction, sequenced and blasted for identification and phylogenetically analyzed. Results and Discussion: The B2L gene sequences of the isolate showed slight variability (96-98.7%) with the reference sequences as it clustered within the same clade with Korean, Zambian and Ethiopian strains, signifying a close genetic relationship. Unique amino acid substitutions were noted. This is the first genetic characterization of B2L gene of orf virus circulating in Nigeria. Conclusion: This study has provided in sight into the genetic diversity of orf virus in the study area. Competing Interests: Financial & competing interests disclosure The authors have no relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript. This includes employment, consultancies, honoraria, stock ownership or options, expert testimony, grants or patents received or pending, or royalties. No writing assistance was utilized in the production of this manuscript. (© 2021 Nafi'u Lawal.)
Databáze:	MEDLINE
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