Plasma-based S100B testing for management of traumatic brain injury in emergency setting.

Autor:	Haselmann V; Department of Clinical Chemistry, University Medicine Mannheim, Medical Faculty Mannheim of the University of Heidelberg, Mannheim, Germany., Schamberger C; Orthopaedic-Trauma Surgery Centre, University Medicine Mannheim, Medical Faculty Mannheim of the University of Heidelberg, Mannheim, Germany., Trifonova F; Department of Clinical Chemistry, University Medicine Mannheim, Medical Faculty Mannheim of the University of Heidelberg, Mannheim, Germany., Ast V; Department of Clinical Chemistry, University Medicine Mannheim, Medical Faculty Mannheim of the University of Heidelberg, Mannheim, Germany., Froelich MF; Institute of Clinical Radiology and Nuclear Medicine, University Medicine Mannheim, Medical Faculty Mannheim of the University of Heidelberg, Mannheim, Germany., Strauß M; Department of Clinical Chemistry, University Medicine Mannheim, Medical Faculty Mannheim of the University of Heidelberg, Mannheim, Germany., Kittel M; Department of Clinical Chemistry, University Medicine Mannheim, Medical Faculty Mannheim of the University of Heidelberg, Mannheim, Germany., Jaruschewski S; Laboratory Diagnostic Center, RWTH University Hospital Aachen, Aachen, Germany., Eschmann D; Orthopaedic-Trauma Surgery Centre, University Medicine Mannheim, Medical Faculty Mannheim of the University of Heidelberg, Mannheim, Germany., Neumaier M; Department of Clinical Chemistry, University Medicine Mannheim, Medical Faculty Mannheim of the University of Heidelberg, Mannheim, Germany., Neumaier-Probst E; Department of Neuroradiology, University Medicine Mannheim, Medical Faculty Mannheim of the University of Heidelberg, Mannheim, Germany.
Jazyk:	angličtina
Zdroj:	Practical laboratory medicine [Pract Lab Med] 2021 May 12; Vol. 26, pp. e00236. Date of Electronic Publication: 2021 May 12 (Print Publication: 2021).
DOI:	10.1016/j.plabm.2021.e00236
Abstrakt:	Background: Serum biomarker S100B has been explored for its potential benefit to improve clinical decision-making in the management of patients suffering from traumatic brain injury (TBI), especially as a pre-head computed-tomography screening test for patients with mild TBI. Although being already included into some guidelines, its implementation into standard care is still lacking. This might be explained by a turnaround time (TAT) too long for serum S100B to be used in clinical decision-making in emergency settings. Methods: S100B concentrations were determined in 136 matching pairs of serum and lithium heparin blood samples. The concordance of the test results was assessed by linear regression, Passing Pablok regression and Bland-Altman analysis. Bias and within- and between-run imprecision were determined by a 5 × 4 model using pooled patient samples. CT scans were performed as clinically indicated. Results: Overall, S100B levels between both blood constituents correlated very well. The suitability of S100B testing from plasma was verified according to ISO15189 requirements. Using a cut-off of 0.105 ng/ml, a sensitivity and negative predictive value of 100% were obtained for identifying patients with pathologic CT scans. Importantly, plasma-based testing reduced the TAT to 26 min allowing for quicker clinical decision-making. The clinical utility of integrating S100B in TBI management is highlighted by two case reports. Conclusions: Plasma-based S100B testing compares favorably with serum-based testing, substantially reducing processing times as the prerequisite for integrating S100B level into management of TBI patients. The proposed new clinical decision algorithm for TBI management needs to be validated in further prospective large-scale studies. Competing Interests: The authors have declared no conflicts of interest. (© 2021 The Authors.)
Databáze:	MEDLINE
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