Large-Scale Recombinant Production of the SARS-CoV-2 Proteome for High-Throughput and Structural Biology Applications.

Autor:	Altincekic N; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Korn SM; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany.; Institute for Molecular Biosciences, Goethe University Frankfurt, Frankfurt am Main, Germany., Qureshi NS; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Dujardin M; Molecular Microbiology and Structural Biochemistry, UMR 5086, CNRS/Lyon University, Lyon, France., Ninot-Pedrosa M; Molecular Microbiology and Structural Biochemistry, UMR 5086, CNRS/Lyon University, Lyon, France., Abele R; Institute for Biochemistry, Goethe University Frankfurt, Frankfurt am Main, Germany., Abi Saad MJ; Swiss Federal Institute of Technology, Laboratory of Physical Chemistry, ETH Zurich, Zurich, Switzerland., Alfano C; Structural Biology and Biophysics Unit, Fondazione Ri.MED, Palermo, Italy., Almeida FCL; National Center of Nuclear Magnetic Resonance (CNRMN, CENABIO), Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.; Institute of Medical Biochemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil., Alshamleh I; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., de Amorim GC; National Center of Nuclear Magnetic Resonance (CNRMN, CENABIO), Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.; Multidisciplinary Center for Research in Biology (NUMPEX), Campus Duque de Caxias Federal University of Rio de Janeiro, Duque de Caxias, Brazil., Anderson TK; Institute for Molecular Virology, University of Wisconsin-Madison, Madison, WI, United States., Anobom CD; National Center of Nuclear Magnetic Resonance (CNRMN, CENABIO), Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.; Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil., Anorma C; Department of Chemistry, University of California, Irvine, CA, United States., Bains JK; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Bax A; LCP, NIDDK, NIH, Bethesda, MD, United States., Blackledge M; Univ. Grenoble Alpes, CNRS, CEA, IBS, Grenoble, France., Blechar J; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Böckmann A; Molecular Microbiology and Structural Biochemistry, UMR 5086, CNRS/Lyon University, Lyon, France., Brigandat L; Molecular Microbiology and Structural Biochemistry, UMR 5086, CNRS/Lyon University, Lyon, France., Bula A; Latvian Institute of Organic Synthesis, Riga, Latvia., Bütikofer M; Swiss Federal Institute of Technology, Laboratory of Physical Chemistry, ETH Zurich, Zurich, Switzerland., Camacho-Zarco AR; Univ. Grenoble Alpes, CNRS, CEA, IBS, Grenoble, France., Carlomagno T; BMWZ and Institute of Organic Chemistry, Leibniz University Hannover, Hannover, Germany.; Group of NMR-Based Structural Chemistry, Helmholtz Centre for Infection Research, Braunschweig, Germany., Caruso IP; National Center of Nuclear Magnetic Resonance (CNRMN, CENABIO), Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.; Institute of Medical Biochemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.; Multiuser Center for Biomolecular Innovation (CMIB), Department of Physics, São Paulo State University (UNESP), São José do Rio Preto, Brazil., Ceylan B; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Chaikuad A; Institute of Pharmaceutical Chemistry, Goethe University Frankfurt, Frankfurt am Main, Germany.; Structural Genomics Consortium, Buchmann Institute for Molecular Life Sciences, Frankfurt am Main, Germany., Chu F; Department of Molecular, Cellular, and Biomedical Sciences, University of New Hampshire, Durham, NH, United States., Cole L; Molecular Microbiology and Structural Biochemistry, UMR 5086, CNRS/Lyon University, Lyon, France., Crosby MG; Department of Molecular Biology and Biochemistry, University of California, Irvine, CA, United States., de Jesus V; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Dhamotharan K; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany.; Institute for Molecular Biosciences, Goethe University Frankfurt, Frankfurt am Main, Germany., Felli IC; Magnetic Resonance Centre (CERM), University of Florence, Sesto Fiorentino, Italy.; Department of Chemistry 'Ugo Schiff', University of Florence, Sesto Fiorentino, Italy., Ferner J; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Fleischmann Y; Swiss Federal Institute of Technology, Laboratory of Physical Chemistry, ETH Zurich, Zurich, Switzerland., Fogeron ML; Molecular Microbiology and Structural Biochemistry, UMR 5086, CNRS/Lyon University, Lyon, France., Fourkiotis NK; Department of Pharmacy, University of Patras, Patras, Greece., Fuks C; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany., Fürtig B; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Gallo A; Department of Pharmacy, University of Patras, Patras, Greece., Gande SL; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Gerez JA; Swiss Federal Institute of Technology, Laboratory of Physical Chemistry, ETH Zurich, Zurich, Switzerland., Ghosh D; Swiss Federal Institute of Technology, Laboratory of Physical Chemistry, ETH Zurich, Zurich, Switzerland., Gomes-Neto F; National Center of Nuclear Magnetic Resonance (CNRMN, CENABIO), Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.; Laboratory of Toxinology, Oswaldo Cruz Foundation (FIOCRUZ), Rio de Janeiro, Brazil., Gorbatyuk O; Department of Molecular Biology and Biophysics, UConn Health, Farmington, CT, United States., Guseva S; Univ. Grenoble Alpes, CNRS, CEA, IBS, Grenoble, France., Hacker C; Signals GmbH & Co. KG, Frankfurt am Main, Germany., Häfner S; Leibniz Institute on Aging-Fritz Lipmann Institute (FLI), Jena, Germany., Hao B; Department of Molecular Biology and Biophysics, UConn Health, Farmington, CT, United States., Hargittay B; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Henzler-Wildman K; Institute for Molecular Virology, University of Wisconsin-Madison, Madison, WI, United States., Hoch JC; Department of Molecular Biology and Biophysics, UConn Health, Farmington, CT, United States., Hohmann KF; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Hutchison MT; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Jaudzems K; Latvian Institute of Organic Synthesis, Riga, Latvia., Jović K; Department of Molecular, Cellular, and Biomedical Sciences, University of New Hampshire, Durham, NH, United States., Kaderli J; Swiss Federal Institute of Technology, Laboratory of Physical Chemistry, ETH Zurich, Zurich, Switzerland., Kalniņš G; Latvian Biomedical Research and Study Centre, Riga, Latvia., Kaņepe I; Latvian Institute of Organic Synthesis, Riga, Latvia., Kirchdoerfer RN; Institute for Molecular Virology, University of Wisconsin-Madison, Madison, WI, United States., Kirkpatrick J; BMWZ and Institute of Organic Chemistry, Leibniz University Hannover, Hannover, Germany.; Group of NMR-Based Structural Chemistry, Helmholtz Centre for Infection Research, Braunschweig, Germany., Knapp S; Institute of Pharmaceutical Chemistry, Goethe University Frankfurt, Frankfurt am Main, Germany.; Structural Genomics Consortium, Buchmann Institute for Molecular Life Sciences, Frankfurt am Main, Germany., Krishnathas R; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Kutz F; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Zur Lage S; Group of NMR-Based Structural Chemistry, Helmholtz Centre for Infection Research, Braunschweig, Germany., Lambertz R; Institute for Molecular Biosciences, Goethe University Frankfurt, Frankfurt am Main, Germany., Lang A; Leibniz Institute on Aging-Fritz Lipmann Institute (FLI), Jena, Germany., Laurents D; 'Rocasolano' Institute for Physical Chemistry (IQFR), Spanish National Research Council (CSIC), Madrid, Spain., Lecoq L; Molecular Microbiology and Structural Biochemistry, UMR 5086, CNRS/Lyon University, Lyon, France., Linhard V; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Löhr F; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany.; Institute of Biophysical Chemistry, Goethe University Frankfurt, Frankfurt am Main, Germany., Malki A; Univ. Grenoble Alpes, CNRS, CEA, IBS, Grenoble, France., Bessa LM; Univ. Grenoble Alpes, CNRS, CEA, IBS, Grenoble, France., Martin RW; Department of Chemistry, University of California, Irvine, CA, United States.; Department of Molecular Biology and Biochemistry, University of California, Irvine, CA, United States., Matzel T; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Maurin D; Univ. Grenoble Alpes, CNRS, CEA, IBS, Grenoble, France., McNutt SW; Department of Molecular, Cellular, and Biomedical Sciences, University of New Hampshire, Durham, NH, United States., Mebus-Antunes NC; National Center of Nuclear Magnetic Resonance (CNRMN, CENABIO), Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.; Institute of Medical Biochemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil., Meier BH; Swiss Federal Institute of Technology, Laboratory of Physical Chemistry, ETH Zurich, Zurich, Switzerland., Meiser N; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany., Mompeán M; 'Rocasolano' Institute for Physical Chemistry (IQFR), Spanish National Research Council (CSIC), Madrid, Spain., Monaca E; Structural Biology and Biophysics Unit, Fondazione Ri.MED, Palermo, Italy., Montserret R; Molecular Microbiology and Structural Biochemistry, UMR 5086, CNRS/Lyon University, Lyon, France., Mariño Perez L; Univ. Grenoble Alpes, CNRS, CEA, IBS, Grenoble, France., Moser C; IBG-4, Karlsruhe Institute of Technology, Karlsruhe, Germany., Muhle-Goll C; IBG-4, Karlsruhe Institute of Technology, Karlsruhe, Germany., Neves-Martins TC; National Center of Nuclear Magnetic Resonance (CNRMN, CENABIO), Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.; Institute of Medical Biochemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil., Ni X; Institute of Pharmaceutical Chemistry, Goethe University Frankfurt, Frankfurt am Main, Germany.; Structural Genomics Consortium, Buchmann Institute for Molecular Life Sciences, Frankfurt am Main, Germany., Norton-Baker B; Department of Chemistry, University of California, Irvine, CA, United States., Pierattelli R; Magnetic Resonance Centre (CERM), University of Florence, Sesto Fiorentino, Italy.; Department of Chemistry 'Ugo Schiff', University of Florence, Sesto Fiorentino, Italy., Pontoriero L; Magnetic Resonance Centre (CERM), University of Florence, Sesto Fiorentino, Italy.; Department of Chemistry 'Ugo Schiff', University of Florence, Sesto Fiorentino, Italy., Pustovalova Y; Department of Molecular Biology and Biophysics, UConn Health, Farmington, CT, United States., Ohlenschläger O; Leibniz Institute on Aging-Fritz Lipmann Institute (FLI), Jena, Germany., Orts J; Swiss Federal Institute of Technology, Laboratory of Physical Chemistry, ETH Zurich, Zurich, Switzerland., Da Poian AT; Institute of Medical Biochemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil., Pyper DJ; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Richter C; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Riek R; Swiss Federal Institute of Technology, Laboratory of Physical Chemistry, ETH Zurich, Zurich, Switzerland., Rienstra CM; Department of Biochemistry and National Magnetic Resonance Facility at Madison, University of Wisconsin-Madison, Madison, WI, United States., Robertson A; LCP, NIDDK, NIH, Bethesda, MD, United States., Pinheiro AS; National Center of Nuclear Magnetic Resonance (CNRMN, CENABIO), Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.; Institute of Chemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil., Sabbatella R; Structural Biology and Biophysics Unit, Fondazione Ri.MED, Palermo, Italy., Salvi N; Univ. Grenoble Alpes, CNRS, CEA, IBS, Grenoble, France., Saxena K; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Schulte L; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Schiavina M; Magnetic Resonance Centre (CERM), University of Florence, Sesto Fiorentino, Italy.; Department of Chemistry 'Ugo Schiff', University of Florence, Sesto Fiorentino, Italy., Schwalbe H; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Silber M; IBG-4, Karlsruhe Institute of Technology, Karlsruhe, Germany., Almeida MDS; National Center of Nuclear Magnetic Resonance (CNRMN, CENABIO), Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.; Institute of Medical Biochemistry, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil., Sprague-Piercy MA; Department of Molecular Biology and Biochemistry, University of California, Irvine, CA, United States., Spyroulias GA; Department of Pharmacy, University of Patras, Patras, Greece., Sreeramulu S; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Tants JN; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany.; Institute for Molecular Biosciences, Goethe University Frankfurt, Frankfurt am Main, Germany., Tārs K; Latvian Biomedical Research and Study Centre, Riga, Latvia., Torres F; Swiss Federal Institute of Technology, Laboratory of Physical Chemistry, ETH Zurich, Zurich, Switzerland., Töws S; Institute for Molecular Biosciences, Goethe University Frankfurt, Frankfurt am Main, Germany., Treviño MÁ; 'Rocasolano' Institute for Physical Chemistry (IQFR), Spanish National Research Council (CSIC), Madrid, Spain., Trucks S; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany., Tsika AC; Department of Pharmacy, University of Patras, Patras, Greece., Varga K; Department of Molecular, Cellular, and Biomedical Sciences, University of New Hampshire, Durham, NH, United States., Wang Y; BMWZ and Institute of Organic Chemistry, Leibniz University Hannover, Hannover, Germany., Weber ME; Swiss Federal Institute of Technology, Laboratory of Physical Chemistry, ETH Zurich, Zurich, Switzerland., Weigand JE; Department of Biology, Technical University of Darmstadt, Darmstadt, Germany., Wiedemann C; Institute of Biochemistry and Biotechnology, Charles Tanford Protein Centre, Martin Luther University Halle-Wittenberg, Halle/Saale, Germany., Wirmer-Bartoschek J; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Wirtz Martin MA; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany.; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany., Zehnder J; Swiss Federal Institute of Technology, Laboratory of Physical Chemistry, ETH Zurich, Zurich, Switzerland., Hengesbach M; Institute for Organic Chemistry and Chemical Biology, Goethe University Frankfurt, Frankfurt am Main, Germany., Schlundt A; Center of Biomolecular Magnetic Resonance (BMRZ), Goethe University Frankfurt, Frankfurt am Main, Germany.; Institute for Molecular Biosciences, Goethe University Frankfurt, Frankfurt am Main, Germany.
Jazyk:	angličtina
Zdroj:	Frontiers in molecular biosciences [Front Mol Biosci] 2021 May 10; Vol. 8, pp. 653148. Date of Electronic Publication: 2021 May 10 (Print Publication: 2021).
DOI:	10.3389/fmolb.2021.653148
Abstrakt:	The highly infectious disease COVID-19 caused by the Betacoronavirus SARS-CoV-2 poses a severe threat to humanity and demands the redirection of scientific efforts and criteria to organized research projects. The international COVID19-NMR consortium seeks to provide such new approaches by gathering scientific expertise worldwide. In particular, making available viral proteins and RNAs will pave the way to understanding the SARS-CoV-2 molecular components in detail. The research in COVID19-NMR and the resources provided through the consortium are fully disclosed to accelerate access and exploitation. NMR investigations of the viral molecular components are designated to provide the essential basis for further work, including macromolecular interaction studies and high-throughput drug screening. Here, we present the extensive catalog of a holistic SARS-CoV-2 protein preparation approach based on the consortium's collective efforts. We provide protocols for the large-scale production of more than 80% of all SARS-CoV-2 proteins or essential parts of them. Several of the proteins were produced in more than one laboratory, demonstrating the high interoperability between NMR groups worldwide. For the majority of proteins, we can produce isotope-labeled samples of HSQC-grade. Together with several NMR chemical shift assignments made publicly available on covid19-nmr.com , we here provide highly valuable resources for the production of SARS-CoV-2 proteins in isotope-labeled form. Competing Interests: CH was employed by Signals GmbH & Co. KG. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2021 Altincekic, Korn, Qureshi, Dujardin, Ninot-Pedrosa, Abele, Abi Saad, Alfano, Almeida, Alshamleh, de Amorim, Anderson, Anobom, Anorma, Bains, Bax, Blackledge, Blechar, Böckmann, Brigandat, Bula, Bütikofer, Camacho-Zarco, Carlomagno, Caruso, Ceylan, Chaikuad, Chu, Cole, Crosby, de Jesus, Dhamotharan, Felli, Ferner, Fleischmann, Fogeron, Fourkiotis, Fuks, Fürtig, Gallo, Gande, Gerez, Ghosh, Gomes-Neto, Gorbatyuk, Guseva, Hacker, Häfner, Hao, Hargittay, Henzler-Wildman, Hoch, Hohmann, Hutchison, Jaudzems, Jović, Kaderli, Kalniņš, Kaņepe, Kirchdoerfer, Kirkpatrick, Knapp, Krishnathas, Kutz, zur Lage, Lambertz, Lang, Laurents, Lecoq, Linhard, Löhr, Malki, Bessa, Martin, Matzel, Maurin, McNutt, Mebus-Antunes, Meier, Meiser, Mompeán, Monaca, Montserret, Mariño Perez, Moser, Muhle-Goll, Neves-Martins, Ni, Norton-Baker, Pierattelli, Pontoriero, Pustovalova, Ohlenschläger, Orts, Da Poian, Pyper, Richter, Riek, Rienstra, Robertson, Pinheiro, Sabbatella, Salvi, Saxena, Schulte, Schiavina, Schwalbe, Silber, Almeida, Sprague-Piercy, Spyroulias, Sreeramulu, Tants, Tārs, Torres, Töws, Treviño, Trucks, Tsika, Varga, Wang, Weber, Weigand, Wiedemann, Wirmer-Bartoschek, Wirtz Martin, Zehnder, Hengesbach and Schlundt.)
Databáze:	MEDLINE
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