Detecting Borrelia Spirochetes: A Case Study With Validation Among Autopsy Specimens.

Autor:	Gadila SKG; Division of Immunology, Tulane National Primate Research Center, Tulane University Health Sciences, Covington, LA, United States., Rosoklija G; Department of Psychiatry, Columbia University, New York, NY, United States.; Division of Molecular Imaging and Neuropathology, New York State Psychiatric Institute, New York, NY, United States., Dwork AJ; Department of Psychiatry, Columbia University, New York, NY, United States.; Division of Molecular Imaging and Neuropathology, New York State Psychiatric Institute, New York, NY, United States.; Macedonian Academy of Sciences and Arts, Skopje, Macedonia.; Department of Pathology and Cell Biology, Columbia University, New York, NY, United States., Fallon BA; Department of Psychiatry, Columbia University, New York, NY, United States., Embers ME; Division of Immunology, Tulane National Primate Research Center, Tulane University Health Sciences, Covington, LA, United States.
Jazyk:	angličtina
Zdroj:	Frontiers in neurology [Front Neurol] 2021 May 10; Vol. 12, pp. 628045. Date of Electronic Publication: 2021 May 10 (Print Publication: 2021).
DOI:	10.3389/fneur.2021.628045
Abstrakt:	The complex etiology of neurodegenerative disease has prompted studies on multiple mechanisms including genetic predisposition, brain biochemistry, immunological responses, and microbial insult. In particular, Lyme disease is often associated with neurocognitive impairment with variable manifestations between patients. We sought to develop methods to reliably detect Borrelia burgdorferi , the spirochete bacteria responsible for Lyme disease, in autopsy specimens of patients with a history of neurocognitive disease. In this report, we describe the use of multiple molecular detection techniques for this pathogen and its application to a case study of a Lyme disease patient. The patient had a history of Lyme disease, was treated with antibiotics, and years later developed chronic symptoms including dementia. The patient's pathology and clinical case description was consistent with Lewy body dementia. B. burgdorferi was identified by PCR in several CNS tissues and by immunofluorescent staining in the spinal cord. These studies offer proof of the principle that persistent infection with the Lyme disease spirochete may have lingering consequences on the CNS. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2021 Gadila, Rosoklija, Dwork, Fallon and Embers.)
Databáze:	MEDLINE
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