Culturing Human Gut Microbiomes in the Laboratory.

Autor:	Renwick S; Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario N1G 2W1, Canada; email: eav@uoguelph.ca, srenwick@uoguelph.ca, ganobisc@uoguelph.ca, elderr@uoguelph.ca, cgianett@uoguelph.ca, higginsg@uoguelph.ca, arobin17@uoguelph.ca, svancure@uoguelph.ca, jwilde@uoguelph.ca., Ganobis CM; Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario N1G 2W1, Canada; email: eav@uoguelph.ca, srenwick@uoguelph.ca, ganobisc@uoguelph.ca, elderr@uoguelph.ca, cgianett@uoguelph.ca, higginsg@uoguelph.ca, arobin17@uoguelph.ca, svancure@uoguelph.ca, jwilde@uoguelph.ca., Elder RA; Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario N1G 2W1, Canada; email: eav@uoguelph.ca, srenwick@uoguelph.ca, ganobisc@uoguelph.ca, elderr@uoguelph.ca, cgianett@uoguelph.ca, higginsg@uoguelph.ca, arobin17@uoguelph.ca, svancure@uoguelph.ca, jwilde@uoguelph.ca., Gianetto-Hill C; Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario N1G 2W1, Canada; email: eav@uoguelph.ca, srenwick@uoguelph.ca, ganobisc@uoguelph.ca, elderr@uoguelph.ca, cgianett@uoguelph.ca, higginsg@uoguelph.ca, arobin17@uoguelph.ca, svancure@uoguelph.ca, jwilde@uoguelph.ca., Higgins G; Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario N1G 2W1, Canada; email: eav@uoguelph.ca, srenwick@uoguelph.ca, ganobisc@uoguelph.ca, elderr@uoguelph.ca, cgianett@uoguelph.ca, higginsg@uoguelph.ca, arobin17@uoguelph.ca, svancure@uoguelph.ca, jwilde@uoguelph.ca., Robinson AV; Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario N1G 2W1, Canada; email: eav@uoguelph.ca, srenwick@uoguelph.ca, ganobisc@uoguelph.ca, elderr@uoguelph.ca, cgianett@uoguelph.ca, higginsg@uoguelph.ca, arobin17@uoguelph.ca, svancure@uoguelph.ca, jwilde@uoguelph.ca., Vancuren SJ; Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario N1G 2W1, Canada; email: eav@uoguelph.ca, srenwick@uoguelph.ca, ganobisc@uoguelph.ca, elderr@uoguelph.ca, cgianett@uoguelph.ca, higginsg@uoguelph.ca, arobin17@uoguelph.ca, svancure@uoguelph.ca, jwilde@uoguelph.ca., Wilde J; Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario N1G 2W1, Canada; email: eav@uoguelph.ca, srenwick@uoguelph.ca, ganobisc@uoguelph.ca, elderr@uoguelph.ca, cgianett@uoguelph.ca, higginsg@uoguelph.ca, arobin17@uoguelph.ca, svancure@uoguelph.ca, jwilde@uoguelph.ca., Allen-Vercoe E; Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario N1G 2W1, Canada; email: eav@uoguelph.ca, srenwick@uoguelph.ca, ganobisc@uoguelph.ca, elderr@uoguelph.ca, cgianett@uoguelph.ca, higginsg@uoguelph.ca, arobin17@uoguelph.ca, svancure@uoguelph.ca, jwilde@uoguelph.ca.
Jazyk:	angličtina
Zdroj:	Annual review of microbiology [Annu Rev Microbiol] 2021 Oct 08; Vol. 75, pp. 49-69. Date of Electronic Publication: 2021 May 26.
DOI:	10.1146/annurev-micro-031021-084116
Abstrakt:	The human gut microbiota is a complex community of prokaryotic and eukaryotic microbes and viral particles that is increasingly associated with many aspects of host physiology and health. However, the classical microbiology approach of axenic culture cannot provide a complete picture of the complex interactions between microbes and their hosts in vivo. As such, recently there has been much interest in the culture of gut microbial ecosystems in the laboratory as a strategy to better understand their compositions and functions. In this review, we discuss the model platforms and methods available in the contemporary microbiology laboratory to study human gut microbiomes, as well as current knowledge surrounding the isolation of human gut microbes for the potential construction of defined communities for use in model systems.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu