| Autor: |
Maria Ranieri A; School of Molecular and Life Sciences, Curtin University, Bentley 6102, WA, Australia. m.massi@curtin.edu.au m.ogden@curtin.edu.au., Vezzelli M; Dipartimento di Scienze Chimiche e Geologiche, Università degli Studi di Modena e Reggio Emilia, via G. Campi 103, 41125 Modena, Italy., Leslie KG; School of Chemistry, The University of Sydney, 2006 NSW, Australia. elizabeth.new@sydney.edu.au., Huang S; Centre for Microscopy, Characterisation and Analysis, Univsersity of Western Australia, 6009 Perth, WA, Australia., Stagni S; Dipartimento di Chimica Industriale 'Toso Montanari', Università degli Studi di Bologna, viale del Risorgimento 4, 40136 Bologna, Italy., Jacquemin D; Laboratoire CEISAM, UMR CNRS 6230, Universit8 de Nantes, 2 Rue de la HoussiniHre, BP 92208, 44322 Nantes Cedex 3, France., Jiang H; Centre for Microscopy, Characterisation and Analysis, Univsersity of Western Australia, 6009 Perth, WA, Australia., Hubbard A; Centre for Microscopy, Characterisation and Analysis, Univsersity of Western Australia, 6009 Perth, WA, Australia., Rigamonti L; Dipartimento di Scienze Chimiche e Geologiche, Università degli Studi di Modena e Reggio Emilia, via G. Campi 103, 41125 Modena, Italy., Watkin ELJ; Curtin Medical School, Curtin University, Kent Street, Bentley 6102 WA, Australia., Ogden MI; School of Molecular and Life Sciences, Curtin University, Bentley 6102, WA, Australia. m.massi@curtin.edu.au m.ogden@curtin.edu.au., New EJ; School of Chemistry, The University of Sydney, 2006 NSW, Australia. elizabeth.new@sydney.edu.au., Massi M; School of Molecular and Life Sciences, Curtin University, Bentley 6102, WA, Australia. m.massi@curtin.edu.au m.ogden@curtin.edu.au. |
| Abstrakt: |
There is a lack of molecular probes for imaging bacteria, in comparison to the array of such tools available for the imaging of mammalian cells. Here, organometallic molecular probes have been developed and assessed for bacterial imaging, designed to have the potential to support multiple imaging modalities. The chemical structure of the probes is designed around a metal-naphthalimide structure. The 4-amino-1,8-naphthalimide moiety, covalently appended through a pyridine ancillary ligand, acts as a luminescent probe for super-resolution microscopy. On the other hand, the metal centre, rhenium(i) or platinum(ii) in the current study, enables techniques such as nanoSIMS. While the rhenium(i) complex was not sufficiently stable to be used as a probe, the platinum(ii) analogue showed good chemical and biological stability. Structured illumination microscopy (SIM) imaging on live Bacillus cereus confirmed the suitability of the probe for super-resolution microscopy. NanoSIMS analysis was used to monitor the uptake of the platinum(ii) complex within the bacteria and demonstrate the potential of this chemical architecture to enable multimodal imaging. The successful combination of these two moieties introduces a platform that could lead to a versatile range of multi-functional probes for bacteria. |
