Harenicola maris gen. nov., sp. nov. isolated from the Sea of Japan shallow sediments.

Autor: Romanenko LA; G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Prospect 100 Let Vladivostoku, 159, Vladivostok, 690022, Russia. lro@piboc.dvo.ru., Kurilenko VV; G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Prospect 100 Let Vladivostoku, 159, Vladivostok, 690022, Russia., Chernysheva NY; G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Prospect 100 Let Vladivostoku, 159, Vladivostok, 690022, Russia., Tekutyeva LA; Innovative Technology Center, Far Eastern Federal University, 8 Suhanova St, Vladivostok, 690950, Russia., Velansky PV; Zhirmunsky Institute of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, 690041, Russia., Svetashev VI; Zhirmunsky Institute of Marine Biology, Far Eastern Branch, Russian Academy of Sciences, Vladivostok, 690041, Russia., Isaeva MP; G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, Prospect 100 Let Vladivostoku, 159, Vladivostok, 690022, Russia.
Jazyk: angličtina
Zdroj: Archives of microbiology [Arch Microbiol] 2021 Sep; Vol. 203 (7), pp. 3973-3979. Date of Electronic Publication: 2021 May 25.
DOI: 10.1007/s00203-021-02360-0
Abstrakt: A Gram-negative, non-motile bacterium КMM 3653 T was isolated from a sediment sample from the Sea of Japan seashore, Russia. On the basis of the 16S rRNA gene sequence analysis the strain КMM 3653 T was positioned within the family Rhodobacteraceae (class Alphaproteobacteria) forming a distinct lineage with the highest gene sequence similarities to the members of the genera Pacificibacter (95.2-94.7%) and Nioella (95.1-94.5%), respectively. According to the phylogenomic tree based on 400 conserved protein sequences, strain КMM 3653 T was placed in the cluster comprising Vannielia litorea, Nioella nitratireducens, Litoreibacter albidus and Pseudoruegeria aquimaris as a separate lineage adjacent to V. litorea KCTC 32083 T . The average nucleotide identity values between strain КMM 3653 T and V. litorea KCTC 32083 T , N. nitratireducens KCTC 32417 T , L. albidus KMM 3851 T , and P. aquimaris CECT 7680 T were 71.1, 70.3, 69.6, and 71.0%, respectively. Strain КMM 3653 T contained Q-10 as the predominant ubiquinone and C 18:1 ω7c as the major fatty acid followed by C 16:0 . The polar lipids were phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, an unidentified phospholipid, two unidentified aminolipids, and five unidentified lipids. The DNA G+C content of 61.8% was calculated from the genome sequence. Based on the phylogenetic evidence and distinctive phenotypic characteristics, we proposed strain KMM 3653 T (= KCTC 82575 T ) to be classified as a novel genus and species Harenicola maris gen. nov., sp. nov.
(© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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