Rapid Screening and Quantitative Determination of Illegal Phosphodiesterase Type 5 Inhibitors (PDE-5i) in Herbal Dietary Supplements.

Autor: Nguyen TO; Vietnam Food Administration, 135 Nui Truc, Ba Dinh, Hanoi 10000, Vietnam.; University of Science, Vietnam National University, 334 Nguyen Trai, Thanh Xuan, Hanoi 10000, Vietnam., Tran CS; National Institute for Food Control, 65 Pham Than Duat, Cau Giay, Hanoi 10000, Vietnam., Do TTH; National Institute for Food Control, 65 Pham Than Duat, Cau Giay, Hanoi 10000, Vietnam., Nguyen TMH; National Institute for Food Control, 65 Pham Than Duat, Cau Giay, Hanoi 10000, Vietnam., Bui QD; National Institute for Food Control, 65 Pham Than Duat, Cau Giay, Hanoi 10000, Vietnam., Bui CT; National Institute for Food Control, 65 Pham Than Duat, Cau Giay, Hanoi 10000, Vietnam., Nguyen HN; National Institute for Food Control, 65 Pham Than Duat, Cau Giay, Hanoi 10000, Vietnam., Dang TH; National Institute for Food Control, 65 Pham Than Duat, Cau Giay, Hanoi 10000, Vietnam., Dinh VC; National Institute for Food Control, 65 Pham Than Duat, Cau Giay, Hanoi 10000, Vietnam., Nguyen TAH; University of Science, Vietnam National University, 334 Nguyen Trai, Thanh Xuan, Hanoi 10000, Vietnam., Le THH; University of Science, Vietnam National University, 334 Nguyen Trai, Thanh Xuan, Hanoi 10000, Vietnam.; National Institute for Food Control, 65 Pham Than Duat, Cau Giay, Hanoi 10000, Vietnam.
Jazyk: angličtina
Zdroj: Journal of analytical methods in chemistry [J Anal Methods Chem] 2021 May 05; Vol. 2021, pp. 5579500. Date of Electronic Publication: 2021 May 05 (Print Publication: 2021).
DOI: 10.1155/2021/5579500
Abstrakt: Phosphodiesterase type 5 inhibitors (PDE-5i) are the first-line medication for oral erectile dysfunction, which are used according to the prescription of doctors. However, these substances have been found illegally in supplementary foods. The quality and safety of dietary supplements for enhancing male sexual performance have been questioned, raising the need for continual development of analytical methods. Liquid chromatography coupled with high-resolution mass spectrometry has become one of the most effective methods to identify and measure PDE-5i concentration. In this research, we focused on (i) developing and validating an effective screening and quantitation method for more than 53 PDE-5i in ingredients and supplementary products using LC-Q-Exactive after a simple sample extraction and (ii) assessing PDE-5i content in natural-based supplementary products available in Vietnam market. The extraction method used a small amount of organic solvent, which makes it more environmentally friendly (greener). The developed method has a limit of detection of 0.4 mg/kg, a limit of quantitation of 1.2 mg/kg, recoveries from 80 to 110%, and repeatability lower than 15%. Ninety-two herbal supplementary foods and ingredients used for enhancement of male sexual performance available in Vietnamese markets were collected. Fourteen PDE-5i including conventional and novel analogous were detected and measured in eighteen food supplements and two formulation ingredient samples.
Competing Interests: The authors declare that there are no conflicts of interest regarding the publication of this paper.
(Copyright © 2021 Thi Oanh Nguyen et al.)
Databáze: MEDLINE
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