Cellular lensing and near infrared fluorescent nanosensor arrays to enable chemical efflux cytometry.

Autor: Cho SY; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA., Gong X; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA., Koman VB; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA., Kuehne M; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA., Moon SJ; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA., Son M; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA., Lew TTS; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.; Institute of Materials Research and Engineering (IMRE), Agency for Science, Technology and Research (A*STAR), Singapore, Singapore., Gordiichuk P; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA., Jin X; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA., Sikes HD; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA., Strano MS; Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA. strano@mit.edu.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2021 May 25; Vol. 12 (1), pp. 3079. Date of Electronic Publication: 2021 May 25.
DOI: 10.1038/s41467-021-23416-1
Abstrakt: Nanosensors have proven to be powerful tools to monitor single cells, achieving spatiotemporal precision even at molecular level. However, there has not been way of extending this approach to statistically relevant numbers of living cells. Herein, we design and fabricate nanosensor array in microfluidics that addresses this limitation, creating a Nanosensor Chemical Cytometry (NCC). nIR fluorescent carbon nanotube array is integrated along microfluidic channel through which flowing cells is guided. We can utilize the flowing cell itself as highly informative Gaussian lenses projecting nIR profiles and extract rich information. This unique biophotonic waveguide allows for quantified cross-correlation of biomolecular information with various physical properties and creates label-free chemical cytometer for cellular heterogeneity measurement. As an example, the NCC can profile the immune heterogeneities of human monocyte populations at attomolar sensitivity in completely non-destructive and real-time manner with rate of ~600 cells/hr, highest range demonstrated to date for state-of-the-art chemical cytometry.
Databáze: MEDLINE
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