Endospore Appendages: a novel pilus superfamily from the endospores of pathogenic Bacilli.

Autor: Pradhan B; Structural and Molecular Microbiology, VIB-VUB Center for Structural Biology, VIB, Brussels, Belgium.; Department of Bioengineering Sciences, Structural Biology Brussels, Vrije Universiteit Brussel, Brussels, Belgium., Liedtke J; Department of Paraclinical Sciences, Faculty of Veterinary Medicine, The Norwegian University of Life Sciences, Ås, Norway., Sleutel M; Structural and Molecular Microbiology, VIB-VUB Center for Structural Biology, VIB, Brussels, Belgium.; Department of Bioengineering Sciences, Structural Biology Brussels, Vrije Universiteit Brussel, Brussels, Belgium., Lindbäck T; Department of Paraclinical Sciences, Faculty of Veterinary Medicine, The Norwegian University of Life Sciences, Ås, Norway., Zegeye ED; Department of Paraclinical Sciences, Faculty of Veterinary Medicine, The Norwegian University of Life Sciences, Ås, Norway., O Sullivan K; Department of Paraclinical Sciences, Faculty of Veterinary Medicine, The Norwegian University of Life Sciences, Ås, Norway., Llarena AK; Department of Paraclinical Sciences, Faculty of Veterinary Medicine, The Norwegian University of Life Sciences, Ås, Norway., Brynildsrud O; Department of Paraclinical Sciences, Faculty of Veterinary Medicine, The Norwegian University of Life Sciences, Ås, Norway.; Division of Infection Control and Environmental Health, Norwegian Institute of Public Health, Oslo, Norway., Aspholm M; Department of Paraclinical Sciences, Faculty of Veterinary Medicine, The Norwegian University of Life Sciences, Ås, Norway., Remaut H; Structural and Molecular Microbiology, VIB-VUB Center for Structural Biology, VIB, Brussels, Belgium.; Department of Bioengineering Sciences, Structural Biology Brussels, Vrije Universiteit Brussel, Brussels, Belgium.
Jazyk: angličtina
Zdroj: The EMBO journal [EMBO J] 2021 Sep 01; Vol. 40 (17), pp. e106887. Date of Electronic Publication: 2021 May 25.
DOI: 10.15252/embj.2020106887
Abstrakt: Bacillus cereus sensu lato is a group of Gram-positive endospore-forming bacteria with high ecological diversity. Their endospores are decorated with micrometer-long appendages of unknown identity and function. Here, we isolate endospore appendages (Enas) from the food poisoning outbreak strain B. cereus NVH 0075-95 and find proteinaceous fibers of two main morphologies: S- and L-Ena. By using cryoEM and 3D helical reconstruction of S-Enas, we show these to represent a novel class of Gram-positive pili. S-Enas consist of single domain subunits with jellyroll topology that are laterally stacked by β-sheet augmentation. S-Enas are longitudinally stabilized by disulfide bonding through N-terminal connector peptides that bridge the helical turns. Together, this results in flexible pili that are highly resistant to heat, drought, and chemical damage. Phylogenomic analysis reveals a ubiquitous presence of the ena-gene cluster in the B. cereus group, which include species of clinical, environmental, and food importance. We propose Enas to represent a new class of pili specifically adapted to the harsh conditions encountered by bacterial spores.
(© 2021 The Authors.)
Databáze: MEDLINE
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